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Article

Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization Assay

1
Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 128442, Jeddah 21362, Saudi Arabia
2
Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80324, Jeddah 21589, Saudi Arabia
3
Resident Preventive Medicine, Ministry of Health (MOH), P.O. Box 109385, Al Madinah Al Munnawarah 21351, Saudi Arabia
*
Author to whom correspondence should be addressed.
Academic Editors: Carmen de Mendoza, Chao-Min Cheng and Sandeep K. Vashist
Diagnostics 2021, 11(6), 994; https://doi.org/10.3390/diagnostics11060994
Received: 16 April 2021 / Revised: 25 May 2021 / Accepted: 28 May 2021 / Published: 30 May 2021
The unusual cases of pneumonia outbreak were reported from Wuhan city in late December 2019. Serological testing provides a powerful tool for the identification of prior infection and for epidemiological studies. Pseudotype virus neutralization assays are widely used for many viruses and applications in the fields of serology. The accuracy of pseudotype neutralizing assay allows for its use in low biosafety lab and provides a safe and effective alternative to the use of wild-type viruses. In this study, we evaluated the performance of this assay compared to the standard microneutralization assay as a reference. The lentiviral pseudotype particles were generated harboring the Spike gene of SARS-CoV-2. The generated pseudotype particles assay was used to evaluate the activity of neutralizing antibodies in 300 human serum samples from a COVID-19 sero-epidemiological study. Testing of these samples resulted in 55 positive samples and 245 negative samples by pseudotype viral particles assay while microneutralization assay resulted in 64 positive and 236 negative by MN assay. Compared to the MN, the pseudotyped viral particles assay showed a sensitivity of 85.94% and a specificity of 100%. Based on the data generated from this study, the pseudotype-based neutralization assay showed a reliable performance for the detection of neutralizing antibodies against SARS-CoV-2 and can be used safely and efficiently as a diagnostic tool in a biosafety level 2 laboratory. View Full-Text
Keywords: SARS-CoV-2; pseudotype; coronavirus; micro-neutralization assay; Saudi Arabia SARS-CoV-2; pseudotype; coronavirus; micro-neutralization assay; Saudi Arabia
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MDPI and ACS Style

Tolah, A.M.K.; Sohrab, S.S.; Tolah, K.M.K.; Hassan, A.M.; El-Kafrawy, S.A.; Azhar, E.I. Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization Assay. Diagnostics 2021, 11, 994. https://doi.org/10.3390/diagnostics11060994

AMA Style

Tolah AMK, Sohrab SS, Tolah KMK, Hassan AM, El-Kafrawy SA, Azhar EI. Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization Assay. Diagnostics. 2021; 11(6):994. https://doi.org/10.3390/diagnostics11060994

Chicago/Turabian Style

Tolah, Ahmed M.K., Sayed S. Sohrab, Khaled M.K. Tolah, Ahmed M. Hassan, Sherif A. El-Kafrawy, and Esam I. Azhar 2021. "Evaluation of a Pseudovirus Neutralization Assay for SARS-CoV-2 and Correlation with Live Virus-Based Micro Neutralization Assay" Diagnostics 11, no. 6: 994. https://doi.org/10.3390/diagnostics11060994

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