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Article

Identification and Quantification of Anti-Gp.Mur Antibodies in Human Serum Using an Insect-Cell-Based System

1
Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Science, National Defense Medical Center, Taipei 115, Taiwan
2
Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan
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Graduate Institute of Life Science, National Defense Medical Center, Taipei 114, Taiwan
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Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taipei 333, Taiwan
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Division of Laboratory Medicine, New Taipei Municipal Tu Cheng Hospital, Tu Cheng, Taipei 236, Taiwan
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Department of Entomology, National Chung Hsing University, Taichung 402, Taiwan
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Department of Plant Pathology and Microbiology, College of Bioresources and Agriculture, National Taiwan University, Taipei 106, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Raul Colodner
Diagnostics 2021, 11(6), 966; https://doi.org/10.3390/diagnostics11060966
Received: 19 March 2021 / Revised: 23 May 2021 / Accepted: 23 May 2021 / Published: 27 May 2021
(This article belongs to the Special Issue Advanced Diagnostic Kit Development for Laboratory Diagnosis)
Gp.Mur is a clinically relevant antigen of the MNS blood group system that is highly prevalent in several Asian populations. Its corresponding antibody, anti-Gp.Mur, has been implicated in hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. Currently, identifying and confirming anti-Gp.Mur antibody presence in sera via agglutination of a panel of red blood cells (RBCs) is inefficient and difficult to quantify. Using a baculovirus expression system to express Gp.Mur antigen on insect cell surfaces, we have developed a quantitative cell-based system to confirm the presence of anti-Gp.Mur antibody in human serum. We obtained 10 serum samples preidentified as having anti-Gp.Mur antibody and another 4 samples containing noncorresponding antibodies from hospital patients. Insect cells displaying Gp.Mur antigen successfully adsorbed anti-Gp.Mur antibody in the sera and inhibited the RBC agglutination mediated by this antibody. By varying the concentration of Gp.Mur-displaying cells, we could grade levels of RBC agglutination by anti-Gp.Mur antibody. Densitometric analysis further enabled quantitative determinations of hemagglutination inhibition by Gp.Mur-displaying cells. We believe that this cell-based hemagglutination inhibition system greatly improves or supplements existing technology and is a convenient means for accurately identifying and quantifying anti-Gp.Mur antibody. View Full-Text
Keywords: baculovirus; blood banking; cell-based assay; Gp.Mur; hemagglutination inhibition baculovirus; blood banking; cell-based assay; Gp.Mur; hemagglutination inhibition
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MDPI and ACS Style

Lamis, R.J.S.; Chiueh, T.-S.; Tsai, C.-H.; Lo, H.-R.; Wei, S.-C.; Chao, Y.-C. Identification and Quantification of Anti-Gp.Mur Antibodies in Human Serum Using an Insect-Cell-Based System. Diagnostics 2021, 11, 966. https://doi.org/10.3390/diagnostics11060966

AMA Style

Lamis RJS, Chiueh T-S, Tsai C-H, Lo H-R, Wei S-C, Chao Y-C. Identification and Quantification of Anti-Gp.Mur Antibodies in Human Serum Using an Insect-Cell-Based System. Diagnostics. 2021; 11(6):966. https://doi.org/10.3390/diagnostics11060966

Chicago/Turabian Style

Lamis, Robert J.S., Tsong-Shi Chiueh, Chih-Hsuan Tsai, Huei-Ru Lo, Sung-Chan Wei, and Yu-Chan Chao. 2021. "Identification and Quantification of Anti-Gp.Mur Antibodies in Human Serum Using an Insect-Cell-Based System" Diagnostics 11, no. 6: 966. https://doi.org/10.3390/diagnostics11060966

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