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Article

Assay System for Simultaneous Detection of SARS-CoV-2 and Other Respiratory Viruses

1
Department of Molecular Diagnostics, Seegene Medical Foundation, Seoul 04805, Korea
2
Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-Associated Disorder Control Technology, Chosun University, Gwangju 61452, Korea
3
Clinical Research Institute, Seegene Medical Foundation, Seoul 04805, Korea
4
Paul F. Glenn Center for Biology of Aging Research, Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA
5
Department of Integrated Biomedical Science and Soonchunhyang Institute of Medi-Bio Science (SIMS), Soonchunhyang University, Cheonan-si 31151, Korea
*
Author to whom correspondence should be addressed.
Equally contributed to this article.
Academic Editor: Renato Millioni
Diagnostics 2021, 11(6), 1084; https://doi.org/10.3390/diagnostics11061084
Received: 12 May 2021 / Revised: 9 June 2021 / Accepted: 10 June 2021 / Published: 13 June 2021
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) triggers disease with nonspecific symptoms that overlap those of infections caused by other seasonal respiratory viruses (RVs), such as the influenza virus (Flu) or respiratory syncytial virus (RSV). A molecular assay for accurate and rapid detection of RV and SARS-CoV-2 is crucial to manage these infections. Here, we compared the analytical performance and clinical reliability of Allplex™ SARS-CoV-2/FluA/FluB/RSV (SC2FabR; Seegene Inc., Seoul, South Korea) kit with those of four commercially available RV detection kits. Upon testing five target viral strains (SARS-CoV-2, FluA, FluB, RSV A, and RSV B), the analytical performance of SC2FabR was similar to that of the other kits, with no significant difference (p ≥ 0.78) in z-scores. The efficiency of SC2FabR (E-value, 81–104%) enabled reliable SARS-CoV-2 and seasonal RV detection in 888 nasopharyngeal swab specimens processed using a fully automated nucleic acid extraction platform. Bland–Altman analyses revealed an agreement value of 95.4% (SD ± 1.96) for the kits, indicating statistically similar results for all five. In conclusion, SC2FabR is a rapid and accurate diagnostic tool for both SARS-CoV-2 and seasonal RV detection, allowing for high-throughput RV analysis with efficiency comparable to that of commercially available kits. This can be used to help manage respiratory infections in patients during and after the coronavirus disease 2019 pandemic. View Full-Text
Keywords: SARS-CoV-2; influenza virus; respiratory syncytial virus; simultaneous detection SARS-CoV-2; influenza virus; respiratory syncytial virus; simultaneous detection
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MDPI and ACS Style

Lim, H.-J.; Park, J.-E.; Park, M.-Y.; Baek, J.-H.; Jung, S.; Sung, N.; Yang, J.-H.; Lee, M.-W.; Lee, S.-H.; Yang, Y.-J. Assay System for Simultaneous Detection of SARS-CoV-2 and Other Respiratory Viruses. Diagnostics 2021, 11, 1084. https://doi.org/10.3390/diagnostics11061084

AMA Style

Lim H-J, Park J-E, Park M-Y, Baek J-H, Jung S, Sung N, Yang J-H, Lee M-W, Lee S-H, Yang Y-J. Assay System for Simultaneous Detection of SARS-CoV-2 and Other Respiratory Viruses. Diagnostics. 2021; 11(6):1084. https://doi.org/10.3390/diagnostics11061084

Chicago/Turabian Style

Lim, Ho-Jae, Jung-Eun Park, Min-Young Park, Joo-Hwan Baek, Sunkyung Jung, Nackmoon Sung, Jae-Hyun Yang, Min-Woo Lee, Sun-Hwa Lee, and Yong-Jin Yang. 2021. "Assay System for Simultaneous Detection of SARS-CoV-2 and Other Respiratory Viruses" Diagnostics 11, no. 6: 1084. https://doi.org/10.3390/diagnostics11061084

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