Next Article in Journal
Hypercoagulability as Measured by Thrombelastography May Be Associated with the Size of Acute Ischemic Infarct—A Pilot Study
Previous Article in Journal
Renal Diseases Associated with Hematologic Malignancies and Thymoma in the Absence of Renal Monoclonal Immunoglobulin Deposits
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Diagnostics
Volume 11
Issue 4
10.3390/diagnostics11040711
diagnostics-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Communication

An aMMP-8 Point-of-Care and Questionnaire Based Real-Time Diagnostic Toolkit for Medical Practitioners

by
Ismo T. Räisänen
1,*,
Hanna Lähteenmäki
1,
Shipra Gupta
2,
Andreas Grigoriadis
3,4,
Vaibhav Sahni
5,
Juho Suojanen
6,7,
Hanna Seppänen
8,9,
Taina Tervahartiala
1,
Dimitra Sakellari
4 and
Timo Sorsa
1,10
1
Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, 00014 Helsinki, Finland
2
Unit of Periodontology, Oral Health Sciences Centre, Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh 160012, India
3
Department of Preventive Dentistry, Periodontology and Implant Biology, Dental School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
4
424 General Military Training Hospital, 54124 Thessaloniki, Greece
5
Department of Periodontics, Dr. Harvansh Singh Judge Institute of Dental Sciences & Hospital, Panjab University, Chandigarh 160014, India
6
Cleft Palate and Craniofacial Center, Department of Plastic Surgery, Helsinki University Hospital, 00029 Helsinki, Finland
7
Department of Oral and Maxillo-Facial Surgery, Päijät-Häme Joint Authority for Health and Wellbeing, 15850 Lahti, Finland
8
Department of Surgery, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland
9
Translational Cancer Medicine Research Program, University of Helsinki, 00014 Helsinki, Finland
10
Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, 14104 Huddinge, Sweden
*
Author to whom correspondence should be addressed.
Diagnostics 2021, 11(4), 711; https://doi.org/10.3390/diagnostics11040711
Submission received: 20 March 2021 / Revised: 7 April 2021 / Accepted: 13 April 2021 / Published: 15 April 2021
(This article belongs to the Section Point-of-Care Diagnostics and Devices)
Browse Figure
Versions Notes

Abstract

The aim of this cross-sectional study is to propose an efficient strategy based on biomarkers adjunct with an interview/questionnaire covering risk factors for periodontitis for the identification of undiagnosed periodontitis by medical professionals. Active matrix metalloproteinase (aMMP)-8 levels in mouthrinse were analyzed by a point-of-care (PoC)/chairside lateral-flow immunotest, and salivary total MMP-8, total MMP-9 and calprotectin levels were analyzed by enzyme-linked immunosorbent assays (ELISAs) and active MMP-9 by gelatin zymography for 149 Greek patients. Patients underwent a full-mouth oral health examination for diagnosis according to the 2018 classification system of periodontal diseases. In addition, patient characteristics (risk factors: age, gender, education level, smoking and body mass index) were recorded. Receiver operating curve (ROC) analysis indicated better diagnostic precision to identify undiagnosed periodontitis for oral fluid biomarkers in adjunct with an interview/questionnaire compared with a plain questionnaire (i.e., risk factors): aMMP-8 AUC (95% confidence interval) = 0.834 (0.761−0.906), total MMP-8 = 0.800 (0.722–0.878), active MMP-9 = 0.787 (0.704–0.870), total MMP-9 = 0.773 (0.687−0.858) and calprotectin = 0.773 (0.687–0.858) vs. questionnaire = 0.764 (0.676–0.851). The findings of this study suggest that oral fluid biomarker analysis, such as a rapid aMMP-8 PoC immunotest, could be used as an adjunct to an interview/questionnaire to improve the precision of timely identification of asymptomatic, undiagnosed periodontitis patients by medical professionals. This strategy appears to be viable for referring patients to a dentist for diagnosis and treatment need assessment.
Keywords: periodontitis; diagnostics; point-of-care systems; biomarkers; matrix metalloproteinase 8; preventive medicine; oral health periodontitis; diagnostics; point-of-care systems; biomarkers; matrix metalloproteinase 8; preventive medicine; oral health

Share and Cite

MDPI and ACS Style

Räisänen, I.T.; Lähteenmäki, H.; Gupta, S.; Grigoriadis, A.; Sahni, V.; Suojanen, J.; Seppänen, H.; Tervahartiala, T.; Sakellari, D.; Sorsa, T. An aMMP-8 Point-of-Care and Questionnaire Based Real-Time Diagnostic Toolkit for Medical Practitioners. Diagnostics 2021, 11, 711. https://doi.org/10.3390/diagnostics11040711

AMA Style

Räisänen IT, Lähteenmäki H, Gupta S, Grigoriadis A, Sahni V, Suojanen J, Seppänen H, Tervahartiala T, Sakellari D, Sorsa T. An aMMP-8 Point-of-Care and Questionnaire Based Real-Time Diagnostic Toolkit for Medical Practitioners. Diagnostics. 2021; 11(4):711. https://doi.org/10.3390/diagnostics11040711

Chicago/Turabian Style

Räisänen, Ismo T., Hanna Lähteenmäki, Shipra Gupta, Andreas Grigoriadis, Vaibhav Sahni, Juho Suojanen, Hanna Seppänen, Taina Tervahartiala, Dimitra Sakellari, and Timo Sorsa. 2021. "An aMMP-8 Point-of-Care and Questionnaire Based Real-Time Diagnostic Toolkit for Medical Practitioners" Diagnostics 11, no. 4: 711. https://doi.org/10.3390/diagnostics11040711

APA Style

Räisänen, I. T., Lähteenmäki, H., Gupta, S., Grigoriadis, A., Sahni, V., Suojanen, J., Seppänen, H., Tervahartiala, T., Sakellari, D., & Sorsa, T. (2021). An aMMP-8 Point-of-Care and Questionnaire Based Real-Time Diagnostic Toolkit for Medical Practitioners. Diagnostics, 11(4), 711. https://doi.org/10.3390/diagnostics11040711

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop