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Mobile Laboratory Reveals the Circulation of Dengue Virus Serotype I of Asian Origin in Medina Gounass (Guediawaye), Senegal

Arboviruses and Hemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, BP220 Dakar, Senegal
Epidemiology Unit, Institut Pasteur de Dakar, BP220 Dakar, Senegal
Microbiology and Animal Hygiene, University of Goettingen, D-33077 Goettingen, Germany
Institute of Aquaculture, University of Stirling, Scotland FK9 4LA, UK
Authors to whom correspondence should be addressed.
Diagnostics 2020, 10(6), 408;
Received: 21 May 2020 / Revised: 9 June 2020 / Accepted: 11 June 2020 / Published: 16 June 2020
(This article belongs to the Special Issue Mobile Diagnosis 2.0)
With the growing success of controlling malaria in Sub-Saharan Africa, the incidence of fever due to malaria is in decline, whereas the proportion of patients with non-malaria febrile illness (NMFI) is increasing. Clinical diagnosis of NMFI is hampered by unspecific symptoms, but early diagnosis is a key factor for both better patient care and disease control. The aim of this study was to determine the arboviral aetiologies of NMFI in low resource settings, using a mobile laboratory based on recombinase polymerase amplification (RPA) assays. The panel of tests for this study was expanded to five arboviruses: dengue virus (DENV), zika virus (ZIKV), yellow fever virus (YFV), chikungunya virus (CHIKV), and rift valley fever virus (RVFV). One hundred and four children aged between one month and 115 months were enrolled and screened. Three of the 104 blood samples of children <10 years presented at an outpatient clinic tested positive for DENV. The results were confirmed by RT-PCR, partial sequencing, and non-structural protein 1 (NS1) antigen capture by ELISA (Biorad, France). Phylogenetic analysis of the derived DENV-1 sequences clustered them with sequences of DENV-1 isolated from Guangzhou, China, in 2014. In conclusion, this mobile setup proved reliable for the rapid identification of the causative agent of NMFI, with results consistent with those obtained in the reference laboratory’s settings. View Full-Text
Keywords: fever; NMFI; mobile laboratory; RPA; DENV fever; NMFI; mobile laboratory; RPA; DENV
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Dieng, I.; Hedible, B.G.; Diagne, M.M.; El Wahed, A.A.; Diagne, C.T.; Fall, C.; Richard, V.; Vray, M.; Weidmann, M.; Faye, O.; Sall, A.A.; Faye, O. Mobile Laboratory Reveals the Circulation of Dengue Virus Serotype I of Asian Origin in Medina Gounass (Guediawaye), Senegal. Diagnostics 2020, 10, 408.

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