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Open AccessArticle

Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot Study

by 1,†, 2,3,†, 1, 4, 2,3,* and 4,*
1
Division of Hematology/Oncology, Department of Internal Medicine, Keimyung University Dongsan Hospital, Daegu 42601, Korea
2
Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Korea
3
Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Korea
4
Department of Obstetrics and Gynecology, Keimyung University Dongsan Hospital, Daegu 42601, Korea
*
Authors to whom correspondence should be addressed.
These authors equally contributed to this article.
Diagnostics 2020, 10(4), 249; https://doi.org/10.3390/diagnostics10040249
Received: 17 March 2020 / Revised: 21 April 2020 / Accepted: 21 April 2020 / Published: 23 April 2020
(This article belongs to the Collection Biomarkers in Medicine)
We investigated the size-based isolation and enumeration of circulating tumor cells (CTCs) using a centrifugal microfluidic device equipped with a fluid-assisted separation technology (FAST) disc. We further assessed the correlations among CTCs, cancer antigen-125 (CA125) levels, and clinical course of the disease in a prospective analysis of 47 serial blood samples collected at multiple time-points from 13 ovarian cancer patients. CTCs were isolated from whole blood using the FAST disc and were classified as epithelial cell adhesion molecule (EpCAM)/cytokeratin+, CD45−, and 4′,6-diamidino-2-phenylindole (DAPI)+. Mean CTC count at baseline was 20.2; 84.62% of patients had more than one CTC at baseline and had decreased CTCs counts after surgery and chemotherapy. The CTC counts in eight patients with complete responses were <3. CTC counts were correlated with CA125 levels in three patients without recurrence; they were elevated in three patients with recurrence and normal CA125 concentrations. CTC counts and CA125 levels showed high concordance with directional changes (increasing 71.4%; non-increasing 75.0%). CTC counts showed higher associations with clinical status, sensitivity (100.0% vs. 60.0%), positive predictive value (55.6% vs. 42.9%), and negative predictive value (100.0% vs. 87.5%) than CA125 levels. CTC counts were better associated with treatment response and recurrence than CA125 levels. View Full-Text
Keywords: circulating tumor cells; fluid-assisted separation technology; centrifugal microfluidic device; CA125; ovarian cancer circulating tumor cells; fluid-assisted separation technology; centrifugal microfluidic device; CA125; ovarian cancer
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MDPI and ACS Style

Kim, H.; Lim, M.; Kim, J.Y.; Shin, S.-J.; Cho, Y.-K.; Cho, C.H. Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot Study. Diagnostics 2020, 10, 249. https://doi.org/10.3390/diagnostics10040249

AMA Style

Kim H, Lim M, Kim JY, Shin S-J, Cho Y-K, Cho CH. Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot Study. Diagnostics. 2020; 10(4):249. https://doi.org/10.3390/diagnostics10040249

Chicago/Turabian Style

Kim, Hyera; Lim, Minji; Kim, Jin Y.; Shin, So-Jin; Cho, Yoon-Kyoung; Cho, Chi H. 2020. "Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot Study" Diagnostics 10, no. 4: 249. https://doi.org/10.3390/diagnostics10040249

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