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Article

Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study

1
Department of Radiology, Rigshospitalet, 2100 Copenhagen, Denmark
2
Department of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark
3
Department of Clinical Medicine, University of Copenhagen, 2200 Copenhagen, Denmark
4
Center for Fast Ultrasound Imaging, Department of Health Technology, Technical University of Denmark, 2800 Lyngby, Denmark
5
BK Medical ApS, 2730 Herlev, Denmark
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(11), 862; https://doi.org/10.3390/diagnostics10110862
Received: 29 September 2020 / Revised: 20 October 2020 / Accepted: 21 October 2020 / Published: 22 October 2020
(This article belongs to the Special Issue New Trends in Vascular Imaging)
In vivo monitoring of the microvasculature is relevant since diseases such as diabetes, ischemia, or cancer cause microvascular impairment. Super-resolution ultrasound imaging allows in vivo examination of the microvasculature by detecting and tracking sparsely distributed intravascular microbubbles over a minute-long period. The ability to create detailed images of the renal vasculature of Sprague-Dawley rats using a modified clinical ultrasound platform was investigated in this study. Additionally, we hypothesized that early ischemic damage to the renal microcirculation could be visualized. After a baseline scan of the exposed kidney, 10 rats underwent clamping of the renal vein (n = 5) or artery (n = 5) for 45 min. The kidneys were rescanned at the onset of clamp release and after 60 min of reperfusion. Using a processing pipeline for tissue motion compensation and microbubble tracking, super-resolution images with a very high level of detail were constructed. Image filtration allowed further characterization of the vasculature by isolating specific vessels such as the ascending vasa recta with a 15–20 μm diameter. Using the super-resolution images alone, it was only possible for six assessors to consistently distinguish the healthy renal microvasculature from the microvasculature at the onset of vein clamp release. Future studies will aim at attaining quantitative estimations of alterations in the renal microvascular blood flow using super-resolution ultrasound imaging. View Full-Text
Keywords: super-resolution ultrasound imaging; rats; Sprague-Dawley; kidney; microcirculation; vasa recta; reperfusion injury super-resolution ultrasound imaging; rats; Sprague-Dawley; kidney; microcirculation; vasa recta; reperfusion injury
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MDPI and ACS Style

Andersen, S.B.; Taghavi, I.; Hoyos, C.A.V.; Søgaard, S.B.; Gran, F.; Lönn, L.; Hansen, K.L.; Jensen, J.A.; Nielsen, M.B.; Sørensen, C.M. Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study. Diagnostics 2020, 10, 862. https://doi.org/10.3390/diagnostics10110862

AMA Style

Andersen SB, Taghavi I, Hoyos CAV, Søgaard SB, Gran F, Lönn L, Hansen KL, Jensen JA, Nielsen MB, Sørensen CM. Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study. Diagnostics. 2020; 10(11):862. https://doi.org/10.3390/diagnostics10110862

Chicago/Turabian Style

Andersen, Sofie Bech, Iman Taghavi, Carlos Armando Villagómez Hoyos, Stinne Byrholdt Søgaard, Fredrik Gran, Lars Lönn, Kristoffer Lindskov Hansen, Jørgen Arendt Jensen, Michael Bachmann Nielsen, and Charlotte Mehlin Sørensen. 2020. "Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study" Diagnostics 10, no. 11: 862. https://doi.org/10.3390/diagnostics10110862

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