Phenotypic and Genotypic Analysis of Resistant Helicobacter pylori Strains Isolated from Children with Gastrointestinal Diseases
Abstract
:1. Introduction
2. Materials and Methods
2.1. Clinical Data
2.2. Detection of H. pylori Genomic DNA Fragments
2.3. Classical Sequencing of H. pylori DNA Fragments
2.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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H. pylori Strains | All Patients [Total] (n = 91) | Chronic Gastritis (n = 35) | GERD (n = 14) | Gastric/Duodenal Ulcer (n = 15) | Other * (n = 27) | |||||
---|---|---|---|---|---|---|---|---|---|---|
Male | Female | Male | Female | Male | Female | Male | Female | Male | Female | |
Susceptible to CH | 17 (18.7%) | 12 (13.2%) | 7 (7.7%) | 5 (5.5%) | 3 (3.3%) | 1 (1.1%) | 1 (1.1%) | 0 (0.0%) | 4 (4.4%) | 8 (8.8%) |
Resistant to CH | 25 (27.5%) | 37 (40.7%) | 13 (14.3%) | 10 (11%) | 4 (4.4%) | 6 (6.6%) | 6 (6.6%) | 8 (8.8%) | 5 (5.5%) | 10 (11%) |
p = 0.032 | p > 0.05 | p = 0.01 | p = 0.03 | p > 0.05 | ||||||
Susceptible to CH + MET | 17 (18.7%) | 40 (44%) | 7 (7.7%) | 13 (14.3%) | 3 (3.3%) | 7 (7.7%) | 1 (1.1%) | 8 (8.8%) | 4 (4.4%) | 18 (19.8%) |
Resistant to CH + MET | 25 (27.5%) | 9 (9.9%) | 13 (14.3%) | 2 (2.2%) | 4 (4.4%) | 0 (0%) | 6 (6.6%) | 0 (0%) | 5 (5.5%) | 0 (0%) |
p > 0.05 | p < 0.01 | p < 0.01 | p < 0.01 | p < 0.01 | ||||||
Susceptible to CH + MET + LEV | 42 (46.1%) | 47 (51.6%) | 20 (22%) | 13 (14.3%) | 7 (7.7%) | 7 (7.7%) | 7 (7.7%) | 8 (8.8%) | 9 (9.9%) | 18 (19.8%) |
Resistant to CH + MET + LEV | 0 (0%) | 2 (2.2%) | 0 (0%) | 2 (2.2%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) |
p < 0.01 | p < 0.01 | p > 0.05 | p > 0.05 | p > 0.05 |
H. pylori Strains | Clinical Diagnosis | Significance | |||
---|---|---|---|---|---|
Chronic Gastritis (n = 35) | GERD (n = 14) | Peptic/Duodenal Ulcer (n = 15) | Other * (n = 27) | ||
R/without mutation | 11% (4) | 7% (1) | 0% (0) | 4% (1) | p > 0.05 |
R/A2142G | 8% (3) | 21% (3) | 7% (1) | 0% (0) | |
R/A2143G | 40% (14) | 36% (5) | 80% (12) | 44% (12) | |
R/T2182C | 6% (2) | 7% (1) | 7% (1) | 7% (2) | |
S/without mutation | 23% (8) | 21% (3) | 7% (1) | 33% (9) | |
S/A2143G | 3% (1) | 7% (1) | 0% (0) | 4% (1) | |
S/T2182C | 9% (3) | 0% (0) | 0% (0) | 7% (2) |
H. pylori Strains | Number of Mutations | Number of Analyzed Positions without Mutations | Significance |
---|---|---|---|
MET-sensitive n = 2656 (16 × 166) | 387 | 2269 | p = 0.0361 (Chi2 = 4.3909) |
MET-resistant n = 3154 (19 × 166) | 400 | 2754 |
H. pylori Strains | All Patients | Mutations | Mutations per Patient | Stop Mutations | AA Changes n (%) | AA Changes per Patient |
---|---|---|---|---|---|---|
MET-sensitive | 16 | 387 | 24.2 | 1 | 82 (21%) | 5 |
MET-resistant | 19 | 400 | 21 | 4 | 90 (22.5%) | 4.7 |
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Biernat, M.M.; Bińkowska, A.; Łaczmański, Ł.; Biernat, P.; Krzyżek, P.; Gościniak, G. Phenotypic and Genotypic Analysis of Resistant Helicobacter pylori Strains Isolated from Children with Gastrointestinal Diseases. Diagnostics 2020, 10, 759. https://doi.org/10.3390/diagnostics10100759
Biernat MM, Bińkowska A, Łaczmański Ł, Biernat P, Krzyżek P, Gościniak G. Phenotypic and Genotypic Analysis of Resistant Helicobacter pylori Strains Isolated from Children with Gastrointestinal Diseases. Diagnostics. 2020; 10(10):759. https://doi.org/10.3390/diagnostics10100759
Chicago/Turabian StyleBiernat, Monika Maria, Aldona Bińkowska, Łukasz Łaczmański, Paweł Biernat, Paweł Krzyżek, and Grażyna Gościniak. 2020. "Phenotypic and Genotypic Analysis of Resistant Helicobacter pylori Strains Isolated from Children with Gastrointestinal Diseases" Diagnostics 10, no. 10: 759. https://doi.org/10.3390/diagnostics10100759