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Toward Experimental Evolution with Giant Vesicles

1
Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan
2
Universal Biology Institute, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan
*
Author to whom correspondence should be addressed.
Received: 26 September 2018 / Revised: 30 October 2018 / Accepted: 30 October 2018 / Published: 31 October 2018
(This article belongs to the Special Issue Approaches toward Artificial Cell Construction and Applications)
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Abstract

Experimental evolution in chemical models of cells could reveal the fundamental mechanisms of cells today. Various chemical cell models, water-in-oil emulsions, oil-on-water droplets, and vesicles have been constructed in order to conduct research on experimental evolution. In this review, firstly, recent studies with these candidate models are introduced and discussed with regards to the two hierarchical directions of experimental evolution (chemical evolution and evolution of a molecular self-assembly). Secondly, we suggest giant vesicles (GVs), which have diameters larger than 1 µm, as promising chemical cell models for studying experimental evolution. Thirdly, since technical difficulties still exist in conventional GV experiments, recent developments of microfluidic devices to deal with GVs are reviewed with regards to the realization of open-ended evolution in GVs. Finally, as a future perspective, we link the concept of messy chemistry to the promising, unexplored direction of experimental evolution in GVs. View Full-Text
Keywords: giant vesicle; oil-on-water droplet; water-in-oil emulsion; experimental evolution; microfluidic device; machine assisted experiment giant vesicle; oil-on-water droplet; water-in-oil emulsion; experimental evolution; microfluidic device; machine assisted experiment
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Sugiyama, H.; Toyota, T. Toward Experimental Evolution with Giant Vesicles. Life 2018, 8, 53.

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