N6-Methyladenosine Modification-Related Genes Express Differentially in Sterile Male Cattle-Yaks
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethical Statement
2.2. Animals
2.3. Testes Measurement
2.4. Hematoxylin and Eosin (H&E) Staining Assays
2.5. Reverse-Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) Assays
2.6. Immunohistochemistry (IHC) Assays
2.7. Western Blotting Assays
2.8. Immunofluorescence Assays
2.9. Statistical Analyses
3. Results
3.1. Morphological and Histological Differences in Testes
3.2. Expression of m6A-Associated Genes in the Testicular Tissue
3.3. Protein Expression of m6A-Associated Genes
3.3.1. Western Blotting Assay
3.3.2. Immunohistochemistry (IHC) Assay
3.3.3. Immunofluorescence Assay
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Testicular Length (cm) | Yak | Cattle-Yak |
---|---|---|
1 | 7.3 | 6.4 |
2 | 7.1 | 6 |
3 | 6.6 | 5.5 |
4 | 6.8 | 5.2 |
5 | 7.5 | 6.4 |
6 | 7.6 | 6.1 |
mean ± SD | 7.150 ± 0.3937 | 5.933 ± 0.4885 *** |
Primer Name | Primer Sequences (5′–3′) |
---|---|
METTL14-F | GCAGAAGTTACGTCGACAGC |
METTL14-R | AGGTATCATAGGAAGCCCTGC |
ALKBH5-F | CGTGACTGTGCTCAGTGGATA |
ALKBH5-R | CGGGGTGCATCTAATCTCGT |
FTO-F | GAGCGCGAAGCTAAGAAAAGA |
FTO-R | TCTGTGCATCAAGGATGGCT |
YTHDF2-F | GGCAGTGGGTTCGGTCATAA |
YTHDF2-R | ACCGAAGCTTCTCCAAGACG |
ACTB-F | CACAGGCCTCTCGCCTTC |
ACTB-R | ATCATCCATGGCGAACTGGT |
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Liu, Y.; Chen, L.; Jiang, H.; Wang, H.; Zhang, Y.; Yuan, Z.; Ma, Y. N6-Methyladenosine Modification-Related Genes Express Differentially in Sterile Male Cattle-Yaks. Life 2024, 14, 1155. https://doi.org/10.3390/life14091155
Liu Y, Chen L, Jiang H, Wang H, Zhang Y, Yuan Z, Ma Y. N6-Methyladenosine Modification-Related Genes Express Differentially in Sterile Male Cattle-Yaks. Life. 2024; 14(9):1155. https://doi.org/10.3390/life14091155
Chicago/Turabian StyleLiu, Yuxin, Lili Chen, Hui Jiang, Hongzhuang Wang, Yujiao Zhang, Zhengrong Yuan, and Yi Ma. 2024. "N6-Methyladenosine Modification-Related Genes Express Differentially in Sterile Male Cattle-Yaks" Life 14, no. 9: 1155. https://doi.org/10.3390/life14091155
APA StyleLiu, Y., Chen, L., Jiang, H., Wang, H., Zhang, Y., Yuan, Z., & Ma, Y. (2024). N6-Methyladenosine Modification-Related Genes Express Differentially in Sterile Male Cattle-Yaks. Life, 14(9), 1155. https://doi.org/10.3390/life14091155