Quantitative Standardized Expansion Assay: An Artificial Intelligence-Powered Morphometric Description of Blastocyst Expansion and Zona Thinning Dynamics
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population and Study Design
2.2. IVF and PGT-A Laboratory Protocols
2.3. Blastocyst Expansion AI-Powered Morphometric and Morphodynamic Analysis
2.4. AI-Powered qSEA
2.5. Clinical Simulations
2.6. Statistical Analysis
3. Results
3.1. Aneuploid Blastocysts Were Slower, Expanded Less and Showed a Thicker Zona Pellucida with Respect to Euploid Embryos
3.2. The Zona Pellucida Thinning Process in the 5 h Following the tB Was More Substantial Among Euploid Blastocysts than Aneuploid
3.3. zp-A, emb-A, and zp-T qSEA Were Significantly Associated with Euploid Blastocysts’ Reproductive Competence
3.4. The zp-T qSEA Would Have Ranked a Euploid Blastocyst as Top Quality in Its Cohort in 57% of the Cycles with >1 Biopsied Blastocyst and Both Euploid and Aneuploid Embryos
3.5. In 69% of the Cycles with >1 Biopsied Blastocyst and Both Euploid and Aneuploid Embryos, the zp-T qSEA Ranking Would Be Equal or Better than Embryologist Rankings
3.6. In 46% of the Cases, the zp-A and emb-A qSEAs Would Have Disagreed with the Embryologists in Prioritizing Euploid Blastocysts for Transfer; The zp-T qSEA, Instead, Would Have Disagreed in 60% of the Cases
4. Discussion
4.1. Clinical Implications of the Evidence Produced in This Study
4.2. Basic Science Data Supporting the Evidence Produced in This Study
4.3. Future Perspectives of Molecular Investigations
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Aneuploid N = 1298 | Euploid N = 886 | p-Value | Euploid–No LB N = 315 | Euploid–LB N = 233 | p-Value | |
---|---|---|---|---|---|---|
tSB, mean ± SD, hpi tB, mean ± SD, hpi tEB, mean ± SD, hpi t-biopsy, mean ± SD, hpi | 102.5 ± 10.5 113.0 ± 12.7 120.9 ± 14.5 136.0 ± 15.2 | 100.5 ± 9.6 109.7 ± 11.1 115.9 ± 12.0 131.6 ± 13.7 | p < 0.01 p < 0.01 p < 0.01 p < 0.01 | 100.3 ± 9.7 109.9 ± 11.2 116.7 ± 12.4 132.4 ± 13.9 | 99.0 ± 9.8 107.5 ± 10.9 112.6 ± 11.2 127.1 ± 12.5 | p = 0.10 p = 0.01 p < 0.01 p < 0.01 |
zp-A at tB, mean ± SD, µm2 | 14,288 ± 1257 | 14,168 ± 1119 | p = 0.02 * | 14,124 ± 1071 | 14,263 ± 1231 | p = 0.16 |
zp-A at tEB, mean ± SD, µm2 | 17,435 ± 1828 | 17,417 ± 1955 | p = 0.82 | 17,482 ± 2234 | 17,542 ± 1835 | p = 0.74 |
Ratio tEB/tB, mean ± SD | +22% ± 10% | +23% ± 12% | p = 0.74 * | +24% ± 14% | +23% ± 9% | p = 0.48 |
zp-A at t-biopsy, mean ± SD, µm2 | 24,082 ± 5763 | 25,438 ± 5968 | p < 0.01 * | 25,141 ± 5873 | 25,790 ± 6159 | p = 0.21 |
Ratio t-biopsy/tEB, mean ± SD | +38% ± 31% | +47% ± 33% | p < 0.01 * | +45% ± 33% | +47% ± 33% | p = 0.35 |
Ratio t-biopsy/tB, mean ± SD | +69% ± 39% | +80% ± 41% | p < 0.01 * | +79% ± 42% | +81% ± 42% | p = 0.47 |
emb-A at tB, mean ± SD, µm2 | 13,349 ± 1196 | 13,249 ± 1121 | p = 0.03 * | 13,235 ± 1107 | 13,309 ± 1121 | p = 0.44 |
emb-A at tEB, mean ± SD, µm2 | 16,900 ± 1867 | 16,922 ± 1986 | p = 0.79 | 16,996 ± 2274 | 17,030 ± 1757 | p = 0.85 |
Ratio tEB/tB, mean ± SD | +27% ± 11% | +28% ± 13% | p = 0.28 * | +29% ± 16% | +28% ± 10% | p = 0.64 |
emb-A at t-biopsy, mean ± SD, µm2 | 23,612 ± 5960 | 25,058 ± 6212 | p < 0.01 * | 24,694 ± 6169 | 25,512 ± 6299 | p = 0.13 |
Ratio t-biopsy/tEB, mean ± SD | +40% ± 34% | +48% ± 36% | p < 0.01 * | +46% ± 35% | +50% ± 34% | p = 0.22 |
Ratio t-biopsy/tB, mean ± SD | +77% ± 44% | +90% ± 47% | p < 0.01 * | +87% ± 48% | +92% ± 46% | p = 0.26 |
zp-T at tB, mean ± SD, µm | 16.4 ± 2.9 | 16.2 ± 2.9 | p = 0.25 | 16.5 ± 3.0 | 16.3 ± 3.1 | p = 0.55 |
zp-T at tEB, mean ± SD, µm | 12.9 ± 2.4 | 12.6 ± 2.5 | p = 0.01 * | 12.9 ± 2.5 | 12.8 ± 2.5 | p = 0.54 |
Ratio tEB/tB, mean ± SD | −21% ± 11% | −22% ± 11% | p = 0.53 * | −21% ± 11% | −22% ± 10% | p = 0.90 |
zp-T at t-biopsy, mean ± SD, µm | 8.1 ± 3.2 | 7.1 ± 2.7 | p < 0.01 * | 7.3 ± 2.9 | 6.9 ± 2.5 | p = 0.11 |
Ratio t-biopsy/tEB, mean ± SD | −37% ± 24% | −43% ± 22% | p = 0.01 * | −43% ± 22% | −44% ± 20% | p = 0.24 |
Ratio t-biopsy/tB, mean ± SD | −50% ± 20% | −55% ± 18% | p < 0.01 * | −55% ± 18% | −57% ± 16% | p = 0.21 |
ICM-A at tB, mean ± SD, µm2 | 3458 ± 905 | 3414 ± 902 | p = 0.27 | 3425 ± 953 | 3434 ± 852 | p = 0.91 |
ICM-A at tEB, mean ± SD, µm2 | 3497 ± 1047 | 3460 ± 1066 | p = 0.43 | 3414 ± 1078 | 3468 ± 984 | p = 0.55 |
Ratio tEB/tB, mean ± SD | +5% ± 35% | +5% ± 33% | p = 0.99 | +4% ± 33% | +3% ± 25% | p = 0.65 |
ICM-A at t-biopsy, mean ± SD, µm2 | 3804 ± 1471 | 3727 ± 1469 | p = 0.31 | 3800 ± 1547 | 3541 ± 1212 | p = 0.08 |
Ratio t-biopsy/tEB, mean ± SD | +11% ± 50% | +10% ± 45% | p = 0.42 | +13% ± 46% | +4% ± 36% | p = 0.17 ** |
Ratio t-biopsy/tB, mean ± SD | +12% ± 49% | +12% ± 51% | p = 0.97 | +16% ± 56% | +6% ± 45% | p = 0.08 |
ICM/TE ratio at tB, mean ± SD | 26% ± 7% | 26% ± 7% | p = 0.61 | 26% ± 7% | 26% ± 7% | p = 0.95 |
ICM/TE ratio at tEB, mean ± SD | 21% ± 6% | 21% ± 6% | p = 0.36 | 20% ± 6% | 20% ± 6% | p = 0.50 |
ICM/TE ratio at t-biopsy, mean ± SD | 17% ± 8% | 16% ± 7% | p = 0.97 * | 16% ± 7% | 15% ± 6% | p = 0.47 ** |
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Cimadomo, D.; Trio, S.; Canosi, T.; Innocenti, F.; Saturno, G.; Taggi, M.; Soscia, D.M.; Albricci, L.; Kantor, B.; Dvorkin, M.; et al. Quantitative Standardized Expansion Assay: An Artificial Intelligence-Powered Morphometric Description of Blastocyst Expansion and Zona Thinning Dynamics. Life 2024, 14, 1396. https://doi.org/10.3390/life14111396
Cimadomo D, Trio S, Canosi T, Innocenti F, Saturno G, Taggi M, Soscia DM, Albricci L, Kantor B, Dvorkin M, et al. Quantitative Standardized Expansion Assay: An Artificial Intelligence-Powered Morphometric Description of Blastocyst Expansion and Zona Thinning Dynamics. Life. 2024; 14(11):1396. https://doi.org/10.3390/life14111396
Chicago/Turabian StyleCimadomo, Danilo, Samuele Trio, Tamara Canosi, Federica Innocenti, Gaia Saturno, Marilena Taggi, Daria Maria Soscia, Laura Albricci, Ben Kantor, Michael Dvorkin, and et al. 2024. "Quantitative Standardized Expansion Assay: An Artificial Intelligence-Powered Morphometric Description of Blastocyst Expansion and Zona Thinning Dynamics" Life 14, no. 11: 1396. https://doi.org/10.3390/life14111396
APA StyleCimadomo, D., Trio, S., Canosi, T., Innocenti, F., Saturno, G., Taggi, M., Soscia, D. M., Albricci, L., Kantor, B., Dvorkin, M., Svensson, A., Huang, T., Vaiarelli, A., Gennarelli, G., & Rienzi, L. (2024). Quantitative Standardized Expansion Assay: An Artificial Intelligence-Powered Morphometric Description of Blastocyst Expansion and Zona Thinning Dynamics. Life, 14(11), 1396. https://doi.org/10.3390/life14111396