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Article

Dietary Supplementation with Boswellia serrata, Verbascum thapsus, and Curcuma longa in Show Jumping Horses: Effects on Serum Proteome, Antioxidant Status, and Anti-Inflammatory Gene Expression

by
Daniela Beghelli
1,*,
Lorenzo Zallocco
2,
Cristina Angeloni
3,
Onelia Bistoni
4,
Maurizio Ronci
5,
Clarita Cavallucci
6,
Maria Rosa Mazzoni
7,
Anna Nuccitelli
1,
Chiara Catalano
8,
Silvana Hrelia
3,
Antonio Lucacchini
9 and
Laura Giusti
10,*
1
School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy
2
Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy
3
Department for Life Quality Studies, Alma Mater Studiorum, University of Bologna, 47921 Rimini, Italy
4
Rheumatology Unit, Department of Medicine, University of Perugia, 06126 Perugia, Italy
5
Department of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, 66100 Chieti, Italy
6
Independent Researcher, 06083 Perugia, Italy
7
Department of Pharmacy, University of Pisa, 56126 Pisa, Italy
8
Independent Researcher, 36023 Vicenza, Italy
9
Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, Italy
10
School of Pharmacy, University of Camerino, 62032 Camerino, Italy
*
Authors to whom correspondence should be addressed.
Life 2023, 13(3), 750; https://doi.org/10.3390/life13030750
Submission received: 4 February 2023 / Revised: 27 February 2023 / Accepted: 7 March 2023 / Published: 10 March 2023
(This article belongs to the Section Genomics and Proteomics)
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Abstract

Intense exercise can cause inflammation and oxidative stress due to the production of reactive oxygen species. These pathophysiological processes are interdependent, and each one can induce the other, creating a vicious circle. A placebo-controlled blind study was carried out in show jumping horses (n. 16) to evaluate the effects of a commercial dietary supplement (Dolhorse® N.B.F. Lanes srl, Milan, Italy) containing Verbascum thapsus leaf powder (1.42%), Curcuma longa (14.280 mg/kg), and Boswellia serrata (Roxb ex Colebr) (14.280 mg/kg) extracts. Before and after 10 days of dietary supplementation, blood samples were collected to evaluate the protein levels, antioxidants, and inflammatory responses by proteomic analysis or real-time Reverse Transcriptase-Polymerase Chain Reaction (real-time RT-PCR). A total of 36 protein spots, connected to 29 proteins, were modulated by dietary supplementation, whereas real-time RT-PCR revealed a significant downregulation of proinflammatory cytokines (interleukin 1α (p < 0.05) and interleukin-6 (0.005), toll-like receptor 4 (p < 0.05), and IKBKB (p < 0.05) in supplemented sport horses. Immunoglobulin chains, gelsolin, plasminogen, vitamin D binding protein, apolipoprotein AIV, and filamin B were overexpressed, whereas haptoglobin, α-2-HS-glycoprotein, α2-macroglobulin, afamin, amine oxidase, 60S acidic ribosomal protein, and complement fragments 3, 4, and 7 were reduced. No effect was observed on the antioxidant defense systems. The present results suggest this phytotherapy may reinforce the innate immune responses, thus representing a valid adjuvant to alleviate inflammation, which is a pathophysiological process in sport horses.
Keywords: Boswellia serrata (Roxb ex Colebr); Verbascum thapsus; Curcuma longa; sport horses; serum proteome; oxidative stress; inflammation; immune responses Boswellia serrata (Roxb ex Colebr); Verbascum thapsus; Curcuma longa; sport horses; serum proteome; oxidative stress; inflammation; immune responses

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MDPI and ACS Style

Beghelli, D.; Zallocco, L.; Angeloni, C.; Bistoni, O.; Ronci, M.; Cavallucci, C.; Mazzoni, M.R.; Nuccitelli, A.; Catalano, C.; Hrelia, S.; et al. Dietary Supplementation with Boswellia serrata, Verbascum thapsus, and Curcuma longa in Show Jumping Horses: Effects on Serum Proteome, Antioxidant Status, and Anti-Inflammatory Gene Expression. Life 2023, 13, 750. https://doi.org/10.3390/life13030750

AMA Style

Beghelli D, Zallocco L, Angeloni C, Bistoni O, Ronci M, Cavallucci C, Mazzoni MR, Nuccitelli A, Catalano C, Hrelia S, et al. Dietary Supplementation with Boswellia serrata, Verbascum thapsus, and Curcuma longa in Show Jumping Horses: Effects on Serum Proteome, Antioxidant Status, and Anti-Inflammatory Gene Expression. Life. 2023; 13(3):750. https://doi.org/10.3390/life13030750

Chicago/Turabian Style

Beghelli, Daniela, Lorenzo Zallocco, Cristina Angeloni, Onelia Bistoni, Maurizio Ronci, Clarita Cavallucci, Maria Rosa Mazzoni, Anna Nuccitelli, Chiara Catalano, Silvana Hrelia, and et al. 2023. "Dietary Supplementation with Boswellia serrata, Verbascum thapsus, and Curcuma longa in Show Jumping Horses: Effects on Serum Proteome, Antioxidant Status, and Anti-Inflammatory Gene Expression" Life 13, no. 3: 750. https://doi.org/10.3390/life13030750

APA Style

Beghelli, D., Zallocco, L., Angeloni, C., Bistoni, O., Ronci, M., Cavallucci, C., Mazzoni, M. R., Nuccitelli, A., Catalano, C., Hrelia, S., Lucacchini, A., & Giusti, L. (2023). Dietary Supplementation with Boswellia serrata, Verbascum thapsus, and Curcuma longa in Show Jumping Horses: Effects on Serum Proteome, Antioxidant Status, and Anti-Inflammatory Gene Expression. Life, 13(3), 750. https://doi.org/10.3390/life13030750

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