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Article

MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker

by
Marta Abrisqueta
1,2,
Sonia Cerdido
1,2,
José Sánchez-Beltrán
1,2,
Idoya Martínez-Vicente
1,
Cecilia Herraiz
1,2,
Ana Lambertos
1,2,
Conchi Olivares
1,2,
Arrate Sevilla
3,
Santos Alonso
4,
María Dolores Boyano
3,5,
José Carlos García-Borrón
1,2 and
Celia Jiménez-Cervantes
1,2,*
1
Department of Biochemistry, Molecular Biology and Immunology, School of Medicine, University of Murcia, LAIB Building, Room 1.53, Campus de Ciencias de la Salud, Carretera Buenavista s/n, 30120 Murcia, Spain
2
Biomedical Research Institute of Murcia (Instituto Murciano de Investigación Biosanitaria, IMIB), 30120 Murcia, Spain
3
Department of Cell Biology and Histology, Faculty of Medicine and Nursing, University of Basque Country UPV/EHU, 48940 Leioa, Spain
4
Department of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, UPV/EHU, University of Basque Country UPV/EHU, 48940 Leioa, Spain
5
Biocruces Bizkaia Health Research Institute, 48903 Barakaldo, Spain
*
Author to whom correspondence should be addressed.
Life 2022, 12(8), 1118; https://doi.org/10.3390/life12081118
Submission received: 23 June 2022 / Revised: 15 July 2022 / Accepted: 21 July 2022 / Published: 26 July 2022
(This article belongs to the Section Medical Research)
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Abstract

Mahogunin Ring Finger 1 (MGRN1), a ubiquitin ligase expressed in melanocytes, interacts with the α melanocyte-stimulating hormone receptor, a well-known melanoma susceptibility gene. Previous studies showed that MGRN1 modulates the phenotype of mouse melanocytes and melanoma cells, with effects on pigmentation, shape, and motility. Moreover, MGRN1 knockdown augmented the burden of DNA breaks in mouse cells, indicating that loss of MGRN1 promoted genomic instability. However, data concerning the roles of MGRN1 in human melanoma cells remain scarce. We analyzed MGRN1 knockdown in human melanoma cells. Transient MGRN1 depletion with siRNA or permanent knockdown in human melanoma cells by CRISPR/Cas9 caused an apparently MITF-independent switch to a more dendritic phenotype. Lack of MGRN1 also increased the fraction of human cells in the S phase of the cell cycle and the burden of DNA breaks but did not significantly impair proliferation. Moreover, in silico analysis of publicly available melanoma datasets and estimation of MGRN1 in a cohort of clinical specimens provided preliminary evidence that MGRN1 expression is higher in human melanomas than in normal skin or nevi and pointed to an inverse correlation of MGRN1 expression in human melanoma with patient survival, thus suggesting potential use of MGRN1 as a melanoma biomarker.
Keywords: Mahogunin Ring Finger 1 (MGRN1); melanocytes; melanoma; DNA damage; biomarker Mahogunin Ring Finger 1 (MGRN1); melanocytes; melanoma; DNA damage; biomarker

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MDPI and ACS Style

Abrisqueta, M.; Cerdido, S.; Sánchez-Beltrán, J.; Martínez-Vicente, I.; Herraiz, C.; Lambertos, A.; Olivares, C.; Sevilla, A.; Alonso, S.; Boyano, M.D.; et al. MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker. Life 2022, 12, 1118. https://doi.org/10.3390/life12081118

AMA Style

Abrisqueta M, Cerdido S, Sánchez-Beltrán J, Martínez-Vicente I, Herraiz C, Lambertos A, Olivares C, Sevilla A, Alonso S, Boyano MD, et al. MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker. Life. 2022; 12(8):1118. https://doi.org/10.3390/life12081118

Chicago/Turabian Style

Abrisqueta, Marta, Sonia Cerdido, José Sánchez-Beltrán, Idoya Martínez-Vicente, Cecilia Herraiz, Ana Lambertos, Conchi Olivares, Arrate Sevilla, Santos Alonso, María Dolores Boyano, and et al. 2022. "MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker" Life 12, no. 8: 1118. https://doi.org/10.3390/life12081118

APA Style

Abrisqueta, M., Cerdido, S., Sánchez-Beltrán, J., Martínez-Vicente, I., Herraiz, C., Lambertos, A., Olivares, C., Sevilla, A., Alonso, S., Boyano, M. D., García-Borrón, J. C., & Jiménez-Cervantes, C. (2022). MGRN1 as a Phenotypic Determinant of Human Melanoma Cells and a Potential Biomarker. Life, 12(8), 1118. https://doi.org/10.3390/life12081118

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