Microbiota of the Colonic Diverticula in the Complicated Form of Diverticulitis: A Case Report
Abstract
:1. Introduction
2. Case Report
3. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Karpukhin, O.Y.; Pankratova, Y.S.; Shakirov, R.R.; Cherkashina, M.I. The influence of the COVID-19 pandemic on the diverticular disease complications treatment. Bull. Contemp. Clin. Med. 2022, 15, 33–39. [Google Scholar] [CrossRef]
- Tursi, A.; Scarpignato, C.; Strate, L.L.; Lanas, A.; Kruis, W.; Lahat, A.; Danese, S. Colonic diverticular disease. Nat. Rev. Dis. Prim. 2020, 6, 1–23. [Google Scholar] [CrossRef]
- Wedel, T.; Barrenschee, M.; Lange, C.; Cossais, F.; Böttner, M. Morphologic Basis for Developing Diverticular Disease, Diverticulitis, and Diverticular Bleeding. Visc. Med. 2015, 31, 76–82. [Google Scholar] [CrossRef] [Green Version]
- Ticinesi, A.; Nouvenne, A.; Corrente, V.; Tana, C.; Di Mario, F.; Meschi, T. Diverticular Disease: A Gut Microbiota Perspective. J. Gastrointest. Liver Dis. 2019, 28, 327–337. [Google Scholar] [CrossRef]
- Reitano, E.; Francone, E.; Bona, E.; Follenzi, A.; Gentilli, S. Gut Microbiota Association with Diverticular Disease Pathogenesis and Progression: A Systematic Review. Am. J. Dig. Dis. 2022, 1–9. [Google Scholar] [CrossRef]
- van Rossen, T.M.; Ooijevaar, R.E.; Kuyvenhoven, J.P.; Eck, A.; Bril, H.; Buijsman, R.; Boermeester, M.A.; Stockmann, H.B.A.C.; de Korte, N.; Budding, A.E. Microbiota composition and mucosal immunity in patients with asymptomatic diverticulosis and controls. PLoS ONE 2021, 16, e0256657. [Google Scholar] [CrossRef]
- Jones, R.B.; Fodor, A.A.; Peery, A.F.; Tsilimigras, M.C.B.; Winglee, K.; McCoy, A.; Sioda, M.; Sandler, R.S.; Keku, T.O. An Aberrant Microbiota is not Strongly Associated with Incidental Colonic Diverticulosis. Sci. Rep. 2018, 8, 4951. [Google Scholar] [CrossRef] [Green Version]
- Tursi, A.; Mastromarino, P.; Capobianco, D.; Elisei, W.; Miccheli, A.; Capuani, G.; Tomassini, A.; Campagna, G.; Picchio, M.; Giorgetti, G.; et al. Assessment of Fecal Microbiota and Fecal Metabolome in Symptomatic Uncomplicated Diverticular Disease of the Colon. J. Clin. Gastroenterol. 2016, 50 (Suppl. 1), S9–S12. [Google Scholar] [CrossRef] [Green Version]
- Barbara, G.; Scaioli, E.; Barbaro, M.R.; Biagi, E.; Laghi, L.; Cremon, C.; Marasco, G.; Colecchia, A.; Picone, G.; Salfi, N.; et al. Gut microbiota, metabolome and immune signatures in patients with uncomplicated diverticular disease. Gut 2016, 66, 1252–1261. [Google Scholar] [CrossRef]
- Schieffer, K.M.; Sabey, K.; Wright, J.R.; Toole, D.R.; Drucker, R.; Tokarev, V.; Harris, L.R.; Deiling, S.; Eshelman, M.A.; Hegarty, J.P.; et al. The Microbial Ecosystem Distinguishes Chronically Diseased Tissue from Adjacent Tissue in the Sigmoid Colon of Chronic, Recurrent Diverticulitis Patients. Sci. Rep. 2017, 7, 8467. [Google Scholar] [CrossRef]
- Spiller, R.C.; Sloan, T.J. Do diverticula provide a unique niche for microbiota which can lead to activation of the innate immune system? Gut 2016, 66, 1175–1176. [Google Scholar] [CrossRef]
- Schultz, J.K.; Azhar, N.; Binda, G.A.; Barbara, G.; Biondo, S.; Boermeester, M.A.; Chabok, A.; Consten, E.C.J.; Van Dijk, S.T.; Johanssen, A.; et al. European Society of Coloproctology: Guidelines for the management of diverticular disease of the colon. Color. Dis. 2020, 22, 5–28. [Google Scholar] [CrossRef]
- Yarullina, D.R.; Shafigullin, M.U.; Sakulin, K.A.; Arzamastseva, A.A.; Shaidullov, I.F.; Markelova, M.I.; Grigoryeva, T.V.; Karpukhin, O.Y.; Sitdikova, G.F. Characterization of gut contractility and microbiota in patients with severe chronic constipation. PLoS ONE 2020, 15, e0235985. [Google Scholar] [CrossRef]
- Stojanov, S.; Berlec, A.; Štrukelj, B. The Influence of Probiotics on the Firmicutes/Bacteroidetes Ratio in the Treatment of Obesity and Inflammatory Bowel disease. Microorganisms 2020, 8, 1715. [Google Scholar] [CrossRef]
- McLean, K.; Rosenthal, C.A.; Sengupta, D.; Owens, J.; Cookson, B.T.; Hoffman, N.G.; Salipante, S.J. Improved Species-Level Clinical Identification of Enterobacteriaceae through Broad-Range dnaJ PCR and Sequencing. J. Clin. Microbiol. 2019, 57, e00986-19. [Google Scholar] [CrossRef]
- Piccioni, A.; Franza, L.; Brigida, M.; Zanza, C.; Torelli, E.; Petrucci, M.; Nicolò, R.; Covino, M.; Candelli, M.; Saviano, A.; et al. Gut Microbiota and Acute Diverticulitis: Role of Probiotics in Management of this Delicate Pathophysiological Balance. J. Pers. Med. 2021, 11, 298. [Google Scholar] [CrossRef]
- Moniuszko, A.; Rydzewska, G. The effect of cyclic rifaximin therapy on symptoms of diverticular disease from the perspective of the gastroenterology outpatient clinic: A “real-life” study. Gastroenterol. Rev. 2017, 12, 145–151. [Google Scholar] [CrossRef] [Green Version]
- Caruso, R.; Lo, B.C.; Núñez, G. Host–microbiota interactions in inflammatory bowel disease. Nat. Rev. Immunol. 2020, 20, 411–426. [Google Scholar] [CrossRef]
- MJ, O.G.; Turner, G.A.; Frizelle, F.A. Distinct changes in the colonic microbiome associated with acute diverticulitis. Color. Dis. 2022, 1–11. [Google Scholar] [CrossRef]
- Arumugam, M.; Raes, J.; Pelletier, E.; Le Paslier, D.; Yamada, T.; Mende, D.R.; Fernandes, G.R.; Tap, J.; Bruls, T.; Batto, J.M.; et al. Enterotypes of the human gut microbiome. Nature 2011, 473, 174–180. [Google Scholar] [CrossRef]
- Wu, G.D.; Chen, J.; Hoffmann, C.; Bittinger, K.; Chen, Y.-Y.; Keilbaugh, S.A.; Bewtra, M.; Knights, D.; Walters, W.A.; Knight, R.; et al. Linking long-term dietary patterns with gut microbial enterotypes. Science 2011, 334, 105–108. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Koeth, R.A.; Wang, Z.; Levison, B.S.; Buffa, J.A.; Org, E.; Sheehy, B.T.; Britt, E.B.; Fu, X.; Wu, Y.; Li, L.; et al. Intestinal microbiota metabolism of l-carnitine, a nutrient in red meat, promotes atherosclerosis. Nat. Med. 2013, 19, 576–585. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Roager, H.M.; Licht, T.R.; Poulsen, S.K.; Larsen, T.M.; Bahl, M.I. Microbial Enterotypes, Inferred by the Prevotella-to-Bacteroides Ratio, Remained Stable during a 6-Month Randomized Controlled Diet Intervention with the New Nordic Diet. Appl. Environ. Microbiol. 2014, 80, 1142–1149. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ryan, M.P.; Adley, C.C. Ralstonia spp.: Emerging global opportunistic pathogens. Eur. J. Clin. Microbiol. 2013, 33, 291–304. [Google Scholar] [CrossRef] [Green Version]
- Chen, J.; Wright, K.; Davis, J.M.; Jeraldo, P.; Marietta, E.V.; Murray, J.; Nelson, H.; Matteson, E.L.; Taneja, V. An expansion of rare lineage intestinal microbes characterizes rheumatoid arthritis. Genome Med. 2016, 8, 43. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Mohan, M.; Chow, C.-E.T.; Ryan, C.N.; Chan, L.S.; Dufour, J.; Aye, P.P.; Blanchard, J.; Moehs, C.P.; Sestak, K. Dietary Gluten-Induced Gut Dysbiosis Is Accompanied by Selective Upregulation of microRNAs with Intestinal Tight Junction and Bacteria-Binding Motifs in Rhesus Macaque Model of Celiac Disease. Nutrients 2016, 8, 684. [Google Scholar] [CrossRef]
Groups of Microorganisms, lg CFU/g | Inflamed Diverticulum (ID) | Non-Inflamed Diverticulum (NID) |
---|---|---|
Total bacterial growth of aerobic bacteria | 13.45 | 13.37 |
Total bacterial growth of anaerobic bacteria | 11.90 | 12.18 |
Lactobacilli | 12.15 | 12.29 |
Bifidobacterium spp. | 11.99 | nd |
Enterobacteriaceae | 12.27 | 7.81 |
Salmonella spp., Shigella spp. | nd | nd |
Inflamed Diverticulum (ID) | Non-Inflamed Diverticulum (NID) | |
---|---|---|
Raw reads | 16,176 | 10,605 |
Non-chimeric reads | 15,765 | 10,587 |
Reads with OTU | 15,012 | 8796 |
Alpha-diversity indices | ||
Observed OTUs | 665 | 458 |
Phylogenetic diversity | 41.0 | 32.6 |
Chao1 | 670.4 | 496.6 |
Shannon | 7.3 | 6.1 |
Simpson | 0.98 | 0.92 |
Taxonomy | Relative Abundance, % | |
---|---|---|
Inflamed Diverticulum (ID) | Non-Inflamed Diverticulum (NID) | |
Actinobacteria | 3.6 | 3.2 |
Actinomycetales | 0.3 | 2.0 |
Coriobacteriales | 3.3 | 0.6 |
Coriobacteriaceae | 3.3 | 0.6 |
Collinsella | 2.3 | 0.4 |
Bacteroidetes | 16.6 | 17.3 |
Bacteroidales | 16.6 | 17.3 |
Bacteroidaceae | 8.0 | 5.4 |
Bacteroides | 8.0 | 5.4 |
Prevotellaceae | 5.4 | 9.6 |
Prevotella | 5.4 | 9.6 |
Firmicutes | 69.3 | 43.2 |
Lactobacillales | 2.5 | 5.8 |
Streptococcaceae | 1.6 | 5.5 |
Streptococcus | 1.4 | 5.5 |
Clostridiales | 66.3 | 36.2 |
Lachnospiraceae | 29.0 | 14.8 |
Blautia | 8.7 | 4.4 |
Coprococcus | 4.7 | 1.4 |
Roseburia | 4.7 | 1.4 |
Ruminococcaceae | 23.2 | 14.3 |
Faecalibacterium | 9.2 | 4.9 |
Ruminococcus | 3.0 | 2.1 |
Unknown Ruminococcaceae | 9.9 | 7.0 |
Proteobacteria | 9.5 | 36.0 |
Burkholderiales | 1.3 | 6.3 |
Oxalobacteraceae | 1.0 | 4.3 |
Ralstonia | 1.0 | 4.3 |
Enterobacteriales | 5.4 | 29.3 |
Enterobacteriaceae | 5.4 | 29.3 |
Unknown Enterobacteriaceae | 5.4 | 29.3 |
Pseudomonadales | 1.4 | 0.0 |
Other phyla | 1 | 0.3 |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Yarullina, D.; Pankratova, Y.; Karaseva, O.; Grigoryeva, T.; Karpukhin, O. Microbiota of the Colonic Diverticula in the Complicated Form of Diverticulitis: A Case Report. Life 2022, 12, 2129. https://doi.org/10.3390/life12122129
Yarullina D, Pankratova Y, Karaseva O, Grigoryeva T, Karpukhin O. Microbiota of the Colonic Diverticula in the Complicated Form of Diverticulitis: A Case Report. Life. 2022; 12(12):2129. https://doi.org/10.3390/life12122129
Chicago/Turabian StyleYarullina, Dina, Yuliia Pankratova, Olga Karaseva, Tatiana Grigoryeva, and Oleg Karpukhin. 2022. "Microbiota of the Colonic Diverticula in the Complicated Form of Diverticulitis: A Case Report" Life 12, no. 12: 2129. https://doi.org/10.3390/life12122129