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Article

Toxicity and Anti-Proliferative Properties of Anisomeles indica Ethanol Extract on Cervical Cancer HeLa Cells and Zebrafish Embryos

1
Faculty of Biology, VNU University of Science, Vietnam National University, Hanoi 100000, Vietnam
2
Unit of Therapeutic Chemistry and Pharmacognosy, University of Mons (UMONS), 7000 Mons, Belgium
3
Laboratory for Organogenesis and Regeneration, GIGA-R, Department Life Sciences, University of Liege, 4000 Liege, Belgium
*
Author to whom correspondence should be addressed.
Academic Editor: Balazs Barna
Life 2021, 11(3), 257; https://doi.org/10.3390/life11030257
Received: 27 February 2021 / Revised: 13 March 2021 / Accepted: 18 March 2021 / Published: 20 March 2021
(This article belongs to the Section Pharmaceutical Science)
In this study, we showed that crude extract of Anisomeles indica (AI-EtE) expressed its toxicity to HeLa cells with an IC50 dose of 38.8 µg/mL and to zebrafish embryos with malformations, lethality and hatching inhibition at 72-hpf at doses higher than 75 µg/mL. More interestingly, flow cytometry revealed that AI-EtE significantly promoted the number of cells entering apoptotic. Accordingly, the transcript levels of BAX, CASPASE-8, and CASPASE-3 in the cells treated with AI-EtE at IC50 dose were 1.55-, 1.62-, and 2.45-fold higher than those in the control cells, respectively. Moreover, treatment with AI-EtE caused cell cycle arrest at the G1 phase in a p53-independent manner. Particularly, percentages of AI-EtE-treated cells in G1, S, G2/M were, respectively 85%, 6.7% and 6.4%; while percentages of control cells in G1, S, G2/M were 64%, 15% and 19%, respectively. Consistent with cell cycle arrest, the expressions of CDKN1A and CDNK2A in AI-EtE-treated cells were up-regulated 1.9- and 1.64-fold, respectively. Significantly, treatment with AI-EtE also decreased anchorage-independent growth of HeLa cells. In conclusion, we suggest that Anisomeles indica can be considered as a medicinal plant with a possible use against cervical cancer cells; however, the used dose should be carefully monitored, especially when applying to pregnant women. View Full-Text
Keywords: medicinal plant; Anisomeles indica; HeLa cell; zebrafish; apoptosis; cell cycle arrest; gene expression; colony formation medicinal plant; Anisomeles indica; HeLa cell; zebrafish; apoptosis; cell cycle arrest; gene expression; colony formation
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MDPI and ACS Style

Bich-Loan, N.T.; Kien, K.T.; Thanh, N.L.; Kim-Thanh, N.T.; Huy, N.Q.; The-Hai, P.; Muller, M.; Nachtergael, A.; Duez, P.; Thang, N.D. Toxicity and Anti-Proliferative Properties of Anisomeles indica Ethanol Extract on Cervical Cancer HeLa Cells and Zebrafish Embryos. Life 2021, 11, 257. https://doi.org/10.3390/life11030257

AMA Style

Bich-Loan NT, Kien KT, Thanh NL, Kim-Thanh NT, Huy NQ, The-Hai P, Muller M, Nachtergael A, Duez P, Thang ND. Toxicity and Anti-Proliferative Properties of Anisomeles indica Ethanol Extract on Cervical Cancer HeLa Cells and Zebrafish Embryos. Life. 2021; 11(3):257. https://doi.org/10.3390/life11030257

Chicago/Turabian Style

Bich-Loan, Nguyen T., Kieu T. Kien, Nguyen L. Thanh, Nguyen T. Kim-Thanh, Nguyen Q. Huy, Pham The-Hai, Marc Muller, Amandine Nachtergael, Pierre Duez, and Nguyen D. Thang 2021. "Toxicity and Anti-Proliferative Properties of Anisomeles indica Ethanol Extract on Cervical Cancer HeLa Cells and Zebrafish Embryos" Life 11, no. 3: 257. https://doi.org/10.3390/life11030257

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