Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Isolation of Primary mNSCs and Cell Culture
2.3. Adherent Monolayer Culture and Differentiation of mNSCs
2.4. Immunocytochemistry Analysis
2.5. Western Blotting
2.6. Cell Migration Assay
2.7. Matrigel Assay
2.8. Cell Proliferation Assay
2.9. Cell Survival Assay
2.10. Statistical Analysis
3. Results
3.1. Isolation of Adult NSCs from the Mouse Subventricular Zone
3.2. Characterization of Adult-Derived mNSCs
3.3. WKYMVm Stimulates Migration of Adult mNSC
3.4. WKYMVm Increases the Expansion and Viability of Adult mNSCs
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Kwon, Y.W.; Bae, S.; Jo, Y.S.; Seo, Y.; Yoon, J.H. Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm. Life 2021, 11, 1248. https://doi.org/10.3390/life11111248
Kwon YW, Bae S, Jo YS, Seo Y, Yoon JH. Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm. Life. 2021; 11(11):1248. https://doi.org/10.3390/life11111248
Chicago/Turabian StyleKwon, Yang Woo, Sungwon Bae, Yeon Suk Jo, Youngsuk Seo, and Jong Hyuk Yoon. 2021. "Stimulation of the Migration and Expansion of Adult Mouse Neural Stem Cells by the FPR2-Specific Peptide WKYMVm" Life 11, no. 11: 1248. https://doi.org/10.3390/life11111248