A Single Faecal Microbiota Transplantation Altered the Microbiota of Weaned Pigs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Design and Sample Collection
- FMT: Faecal transplant administered two days post weaning (n = 21; FMT)
- Control: Saline administered two days post weaning (n = 21; CON)
2.2. DNA Extraction and 16S rRNA Amplicon Sequencing
2.3. Statistical Methods
3. Results
3.1. Production Characteristics
3.2. Age Related Effects
3.3. Donor Sample Composition
3.4. Treatment by Age Effects
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Group FMT | Group CON | ||||||
---|---|---|---|---|---|---|---|
Phyla | Class | Order | Family | Genus | Average Abundance | Average Abundance | % |
Actinobacteria | Coriobacteriia | Coriobacteriales | Coriobacteriaceae | Collinsella | 0.26 | 0.27 | 0.69 |
Bacteroidetes | Bacteroidia | Bacteroidales | Bacteroidaceae | Bacteroides | 0.82 | 0.68 | 1.24 |
Odoribacteraceae | Butyricimonas | 0.27 | 0.25 | 0.75 | |||
p-2534-18B5 | 0.46 | 0.29 | 1.09 | ||||
Paraprevotellaceae | YRC22 | 0.61 | 0.38 | 0.94 | |||
CF231 | 0.9 | 0.9 | 0.81 | ||||
Porphyromonadaceae | Parabacteroides | 1.02 | 0.79 | 1.25 | |||
Paludibacter | 0.37 | 0.24 | 1.18 | ||||
Prevotellaceae | Prevotella | 2.12 | 2.31 | 1.13 | |||
Prevotella | 1.19 | 1.03 | 1.09 | ||||
Prevotellaceae | 0.69 | 0.58 | 0.82 | ||||
RF16 | 0.47 | 0.36 | 0.81 | ||||
Rikenellaceae | 0.25 | 0.2 | 0.73 | ||||
S24-7 | 1.77 | 1.64 | 0.99 | ||||
Deferribacteres | Deferribacteres | Deferribacterales | Deferribacteraceae | Mucispirillum | 0.4 | 0.21 | 0.8 |
Elusimicrobia | Elusimicrobia | Elusimicrobiales | Elusimicrobiaceae | 0.26 | 0.12 | 0.77 | |
Fibrobacteres | Fibrobacteria | Fibrobacterales | Fibrobacteraceae | Fibrobacter | 0.36 | 0.22 | 0.7 |
Firmicutes | Bacilli | Lactobacillales | Lactobacillaceae | Lactobacillus | 0.46 | 0.5 | 0.98 |
Streptococcaceae | Streptococcus | 0.41 | 0.32 | 0.78 | |||
Clostridia | Clostridiales | Christensenellaceae | 1.1 | 0.9 | 1.27 | ||
Clostridiaceae | Sarcina | 0.82 | 0.67 | 1.02 | |||
Clostridiaceae | 0.49 | 0.41 | 0.81 | ||||
SMB53 | 0.48 | 0.37 | 0.72 | ||||
Lachnospiraceae | 1.43 | 1.2 | 1.16 | ||||
Shuttleworthia | 0.37 | 0.13 | 1.02 | ||||
Dorea | 0.75 | 0.63 | 0.96 | ||||
Butyrivibrio | 0.54 | 0.4 | 0.94 | ||||
Lachnobacterium | 0.38 | 0.25 | 0.73 | ||||
Roseburia | 0.84 | 0.93 | 0.69 | ||||
Lachnospira | 0.71 | 0.65 | 0.69 | ||||
Mogibacteriaceae | 0.82 | 0.72 | 0.8 | ||||
Peptococcaceae | rc4-4 | 0.33 | 0.26 | 0.66 | |||
Ruminococcaceae | Faecalibacterium | 1.01 | 1.14 | 0.91 | |||
Oscillospira | 1.52 | 1.45 | 0.73 | ||||
Ruminococcaceae | 1.69 | 1.56 | 0.66 | ||||
Veillonellaceae | Mitsuokella | 0.43 | 0.53 | 1.07 | |||
Dialister | 0.54 | 0.2 | 1.06 | ||||
Veillonellaceae | 0.63 | 0.61 | 1 | ||||
Acidaminococcus | 0.57 | 0.43 | 0.98 | ||||
Megasphaera | 0.63 | 0.64 | 0.9 | ||||
Anaerovibrio | 0.95 | 0.91 | 0.75 | ||||
Clostridiales | 1.07 | 0.91 | 1.01 | ||||
Clostridiales | 1.17 | 1.02 | 0.66 | ||||
Firmicutes | Erysipelotrichi | Erysipelotrichales | Erysipelotrichaceae | p-75-a5 | 0.77 | 0.67 | 0.82 |
Catenibacterium | 0.65 | 0.71 | 0.8 | ||||
Proteobacteria | Alphaproteobacteria | RF32 | 0.33 | 0.21 | 0.82 | ||
Alphaproteobacteria | 0.3 | 0.16 | 0.76 | ||||
Deltaproteobacteria | GMD14H09 | 0.72 | 0.68 | 1.1 | |||
Epsilonproteobacteria | Campylobacterales | Campylobacteraceae | Campylobacter | 0.91 | 0.75 | 1.08 | |
Gammaproteobacteria | Aeromonadales | Succinivibrionaceae | Succinivibrio | 1.18 | 1.44 | 1.85 | |
Succinivibrionaceae | Ruminobacter | 0.63 | 0.39 | 1.51 | |||
Succinivibrionaceae | 0.52 | 0.15 | 1.5 | ||||
Enterobacteriales | Enterobacteriaceae | Escherichia | 0.76 | 0.54 | 1.33 | ||
Pasteurellales | Pasteurellaceae | Actinobacillus | 0.37 | 0.3 | 0.76 | ||
Spirochaetes | Spirochaetes | Spirochaetales | Spirochaetaceae | Treponema | 1.31 | 1.16 | 1.14 |
Synergistetes | Synergistia | Synergistales | Dethiosulfovibrionaceae | Pyramidobacter | 0.36 | 0.26 | 0.87 |
Dethiosulfovibrionaceae | 0.33 | 0.27 | 0.66 | ||||
Tenericutes | RF3 | ML615J-28 | 0.38 | 0.27 | 0.75 | ||
TM7 | TM7-3 | CW040 | F16 | 0.33 | 0.17 | 0.8 | |
Verrucomicrobia | Verruco-5 | WCHB1-41 | RFP12 | 0.33 | 0.18 | 0.66 | |
WPS-2 | 0.31 | 0.21 | 0.74 |
Group FMT | Group CON | ||||||
---|---|---|---|---|---|---|---|
Phyla | Class | Order | Family | Genus | Average Abundance | Average Abundance | % |
Actinobacteria | Coriobacteriia | Coriobacteriales | Coriobacteriaceae | Collinsella | 0.37 | 0.28 | 0.77 |
Bacteroidetes | Bacteroidia | Bacteroidales | Paraprevotellaceae | YRC22 | 0.21 | 0.28 | 0.9 |
CF231 | 0.75 | 0.88 | 0.74 | ||||
0.7 | 0.55 | 0.69 | |||||
Porphyromonadaceae | Parabacteroides | 0.52 | 0.59 | 0.94 | |||
Prevotellaceae | Prevotella | 1.19 | 1.3 | 1.12 | |||
S24-7 | 1.32 | 1.3 | 0.82 | ||||
Bacteroidales | 0.43 | 0.41 | 0.9 | ||||
Deferribacteres | Deferribacteres | Deferribacterales | Deferribacteraceae | Mucispirillum | 0.35 | 0.26 | 0.99 |
Elusimicrobia | Elusimicrobia | Elusimicrobiales | Elusimicrobiaceae | 0.21 | 0.07 | 0.79 | |
Fibrobacteres | Fibrobacteria | Fibrobacterales | Fibrobacteraceae | Fibrobacter | 0.31 | 0.2 | 0.78 |
Streptococcaceae | Streptococcus | 0.45 | 0.32 | 1.01 | |||
Firmicutes | Clostridia | Clostridiales | Clostridiaceae | Sarcina | 0.53 | 0.73 | 1.31 |
SMB53 | 0.86 | 0.88 | 0.84 | ||||
Clostridiaceae | 0.94 | 0.95 | 0.82 | ||||
Lachnospiraceae | Shuttleworthia | 0.53 | 0.41 | 1.64 | |||
Lachnobacterium | 0.56 | 0.79 | 1.63 | ||||
Lachnospiraceae | 1.32 | 1.4 | 1.39 | ||||
Roseburia | 1.47 | 1.55 | 1.1 | ||||
Anaerostipes | 0.4 | 0.32 | 0.87 | ||||
Lachnospira | 0.95 | 1.08 | 0.81 | ||||
Ruminococcus | 1.06 | 1.03 | 0.79 | ||||
Dorea | 0.68 | 0.71 | 0.78 | ||||
Coprococcus | 0.94 | 0.96 | 0.72 | ||||
Peptococcaceae | rc4-4 | 0.11 | 0.28 | 0.87 | |||
Ruminococcaceae | Faecalibacterium | 1.18 | 1.08 | 0.82 | |||
Oscillospira | 1.28 | 1.23 | 0.77 | ||||
Veillonellaceae | Dialister | 0.91 | 0.34 | 2.29 | |||
Veillonellaceae | 0.71 | 0.66 | 1.43 | ||||
Acidaminococcus | 0.53 | 0.34 | 1.4 | ||||
Mitsuokella | 0.65 | 0.53 | 1.35 | ||||
Megasphaera | 0.7 | 0.53 | 1.28 | ||||
Phascolarctobacterium | 0.72 | 0.96 | 0.99 | ||||
Clostridia | 0.24 | 0.04 | 0.86 | ||||
Erysipelotrichi | Erysipelotrichales | Erysipelotrichaceae | Catenibacterium | 0.75 | 0.58 | 1.13 | |
Bulleidia | 0.98 | 0.91 | 1.09 | ||||
p-75-a5 | 0.64 | 0.68 | 0.77 | ||||
L7A_E11 | 0.33 | 0.25 | 0.74 | ||||
Proteobacteria | Alphaproteobacteria | RF32 | 0.36 | 0.45 | 1.09 | ||
Betaproteobacteria | Burkholderiales | Oxalobacteraceae | Oxalobacter | 0.14 | 0.15 | 0.73 | |
Tremblayales | 0.32 | 0.26 | 1.44 | ||||
Deltaproteobacteria | GMD14H09 | 0.52 | 0.45 | 1.36 | |||
Epsilonproteobacteria | Campylobacterales | Campylobacteraceae | Campylobacter | 0.77 | 0.67 | 1.34 | |
Gammaproteobacteria | Aeromonadales | Succinivibrionaceae | Succinivibrio | 0.93 | 0.98 | 1.84 | |
Succinivibrionaceae | 0.47 | 0.2 | 1.7 | ||||
Enterobacteriales | Enterobacteriaceae | Escherichia | 0.34 | 0.27 | 0.81 | ||
Pasteurellales | Pasteurellaceae | Actinobacillus | 0.32 | 0.15 | 0.93 | ||
Spirochaetes | Spirochaetes | Spirochaetales | Spirochaetaceae | Treponema | 0.91 | 1.01 | 1.65 |
Synergistetes | Synergistia | Synergistales | Dethiosulfovibrionaceae | 0.22 | 0.27 | 0.7 | |
TM7 | TM7-3 | CW040 | F16 | 0.38 | 0.41 | 0.93 | |
Unclassified Bacteria | 0.26 | 0.14 | 0.81 |
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Nowland, T.L.; Torok, V.A.; Low, W.Y.; Plush, K.J.; Barton, M.D.; Kirkwood, R.N. A Single Faecal Microbiota Transplantation Altered the Microbiota of Weaned Pigs. Life 2020, 10, 203. https://doi.org/10.3390/life10090203
Nowland TL, Torok VA, Low WY, Plush KJ, Barton MD, Kirkwood RN. A Single Faecal Microbiota Transplantation Altered the Microbiota of Weaned Pigs. Life. 2020; 10(9):203. https://doi.org/10.3390/life10090203
Chicago/Turabian StyleNowland, Tanya L., Valeria A. Torok, Wai Y. Low, Kate J. Plush, Mary D. Barton, and Roy N. Kirkwood. 2020. "A Single Faecal Microbiota Transplantation Altered the Microbiota of Weaned Pigs" Life 10, no. 9: 203. https://doi.org/10.3390/life10090203