Applications of Proteomic Tools to Study Insect Vector–Plant Virus Interactions
Abstract
:1. Introduction
2. Proteomic Approaches to Study of Vector–Virus Interactions
2.1. Detection of Infection
2.2. Techniques to Identify Global Changes to the Vector Proteome upon Infection
2.2.1. Mass Spectrometry for Global Proteomics
2.2.2. Sample Collection and Protein Isolation for Global Proteome
2.2.3. Gel-Based Proteomics
2.2.4. Gel-Free Proteomics
2.3. Techniques to Identify Vector Proteins Interacting with Virus Proteins
3. Combining Proteomics with Multiple ‘-Omics’-Based Techniques
4. Bioinformatic Analysis of Proteomics Data
5. Conclusions and Future Directions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Insect Vector | Virus | Antigen | ELISA Type | Reference |
---|---|---|---|---|
Rhopalosiphum padi and Rhopalosiphum maidis | Barley yellow dwarf virus (BYDV) | Antiserum made by injecting PAV and MAV (two isolates transmitted by R. padi) to rabbits | Double antibody sandwich (DAS) ELISA | Lister and Rochow 1979 [23] |
Aphis gossypii | Plum pox potyvirus (PPV)-D and M serotypes | PPV-D- and PPV-M-specific monoclonal antibodies | Double antibody sandwich indirect (DASI) ELISA | Olmos et al. 1997 [24] |
Myzus persicae | Beet yellow virus (BVY) | BVY-specific monoclonal antibody | Triple antibody sandwich (TAS) ELISA | Stevens et al. 1997 [25] |
Myzus persicae | Alfa mosaic virus (AMV) | Glutaraldehyde-fixed AMV antisera raised in rabbits | Indirect double antibody sandwich (IDAS) ELISA | Ahoonmanesh et al. 1990 [26] |
Bemisia tabaci | Lettuce infectious yellows virus (LIYV) | Coat protein (CPm) | Double and triple antibody sandwich (DAS and TAS) ELISA | Chen et al. 2011 [9] |
Peregrinus maidis | Maize stripe virus (MStpV) | Purified non-capsid protein (Falk 1983) | Double antibody sandwich (DAS) ELISA | Falk et al. 1987 [27] |
Peregrinus maidis | Maize stripe tenuivirus (MStV) | MStV-US * [28] | Indirect ELISA | Ammar et al. 1995 [29] |
Cicadulina chinai | Maize yellow stripe virus (MYSV) | MYSV * [30] | Direct antigen coating (DAC) ELISA | Ammar et al. 2007 [31] |
Cicadulia mbila | Maize streak virus (MSV) | MSV | Indirect double antibody sandwich (IDAS) ELISA | Reynaud and Peterschmitt 1992 [32] |
Frankliniella occidentalis | Tomato spotted wilt virus (TSWV) | Nucleocapsid and glycoprotein (GN) | Triple antibody sandwich (TAS) ELISA | Margaria et al. 2014 [33] |
Frankliniella occidentalis | Tomato zonate spot virus (TZSV) | TZSV * | Double antibody sandwich (DAS) ELISA | Yin et al. 2014 [34] |
Bemisia tabaci | Tomato yellow leaf curl Thailand virus (TYLCTHV) | Recombinant coat protein (CP) | Triple antibody sandwich (TAS) ELISA | Seepiban et al. 2017 [35] |
Approaches to Study Protein-Protein Interactions | Pros/Cons | Examples of Insect Vector–Plant Virus System Using the Approach |
---|---|---|
In Vitro Techniques | ||
One- or Two-Dimensional Virus Overlay/Far Western Blot Assays | Pros:
| Myzus persicae–Potato leaf virus [70] Frankliniella occidentalis–Tomato spotted wilt virus [60,71,72] Myzus persicae–Beet western yellow virus [59] Acyrthosiphon pisum–Pea enation mosaic virus I [73] Sitobion avenae–Barley yellow dwarf virus-MAV [74,75] Laodelphax striatellus–Rice stripe virus [69] |
Coimmunoprecipitation | Pros:
| Sogatella furcifera–Southern rice black-streaked dwarf virus [68] |
Cross-Linking/Protein Interaction Reporter Technology | Pros:
| Myzus persicae–Potato leafroll virus [76] |
Protein or Peptide Arrays | Pros:
| Aphis pisum–Cucumber mosaic virus [77] |
In Vivo Techniques | ||
Yeast Two-Hybrid | Pros:
| Bemisia tabaci–Tomato yellow leaf curl sardinia virus [78] Spodoptera furcifera–Southern rice black-streaked dwarf virus [67] Myzus persicae–Cucumber mosaic virus [69] Laodelphax striatellus–Rice stripe virus [66,67,79] Myzus persicae–Turnip yellow virus [80] Frankliniella occidentalis–Tomato spotted wilt virus [60] |
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Mittapelly, P.; Rajarapu, S.P. Applications of Proteomic Tools to Study Insect Vector–Plant Virus Interactions. Life 2020, 10, 143. https://doi.org/10.3390/life10080143
Mittapelly P, Rajarapu SP. Applications of Proteomic Tools to Study Insect Vector–Plant Virus Interactions. Life. 2020; 10(8):143. https://doi.org/10.3390/life10080143
Chicago/Turabian StyleMittapelly, Priyanka, and Swapna Priya Rajarapu. 2020. "Applications of Proteomic Tools to Study Insect Vector–Plant Virus Interactions" Life 10, no. 8: 143. https://doi.org/10.3390/life10080143