Functional Roles of RNA-Binding Proteins in Plant Signaling
1
Department of Biochemistry, Faculty of Medicine, Midlands State University, Main Campus, Senga Road, Gweru P Bag 9055, Zimbabwe
2
Rijk Zwaan, 2678 ZG De Lier, The Netherlands
*
Author to whom correspondence should be addressed.
Life 2020, 10(11), 288; https://doi.org/10.3390/life10110288
Received: 27 October 2020
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Revised: 14 November 2020
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Accepted: 16 November 2020
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Published: 18 November 2020
(This article belongs to the Special Issue Plant Proteomics)
RNA-binding proteins (RBPs) are typical proteins that bind RNA through single or multiple RNA-binding domains (RBDs). These proteins have a functional role in determining the fate or function of the bound RNAs. A few hundred RBPs were known through in silico prediction based on computational assignment informed by structural similarity and the presence of classical RBDs. However, RBPs lacking such conventional RBDs were omitted. Owing to the recent mRNA interactome capture technology based on UV-crosslinking and fixing proteins to their mRNA targets followed by affinity capture purification and identification of RBPs by tandem mass spectrometry, several hundreds of RBPs have recently been discovered. These proteome-wide studies have colossally increased the number of proteins implicated in RNA binding and unearthed hundreds of novel RBPs lacking classical RBDs, such as proteins involved in intermediary metabolism. These discoveries provide wide insights into the post-transcriptional gene regulation players and their role in plant signaling, such as environmental stress conditions. In this review, novel discoveries of RBPs are explored, particularly on the evolving knowledge of their role in stress responses. The molecular functions of these RBPs, particularly focusing on those that do not have classical RBDs, are also elucidated at the systems level.
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Keywords:
RNA-binding protein; RNA interactome capture; signaling; abiotic stress; mass spectrometry
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MDPI and ACS Style
Muleya, V.; Marondedze, C. Functional Roles of RNA-Binding Proteins in Plant Signaling. Life 2020, 10, 288. https://doi.org/10.3390/life10110288
AMA Style
Muleya V, Marondedze C. Functional Roles of RNA-Binding Proteins in Plant Signaling. Life. 2020; 10(11):288. https://doi.org/10.3390/life10110288
Chicago/Turabian StyleMuleya, Victor, and Claudius Marondedze. 2020. "Functional Roles of RNA-Binding Proteins in Plant Signaling" Life 10, no. 11: 288. https://doi.org/10.3390/life10110288
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