Next Article in Journal
Telomere Instability in Lynch Syndrome Families Leads to Some Shorter Telomeres in MSH2+/- Carriers
Next Article in Special Issue
Macrobiont: Cradle for the Origin of Life and Creation of a Biosphere
Previous Article in Journal
Modeling Mucopolysaccharidosis Type II in the Fruit Fly by Using the RNA Interference Approach
Previous Article in Special Issue
The Habitability of the Galactic Bulge
Open AccessArticle

Effects of Heavy Ion Particle Irradiation on Spore Germination of Bacillus spp. from Extremely Hot and Cold Environments

1
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Research Centre for Extreme Environments and Extremophiles, University of Messina, V.le F. Stagno d’Alcontres 31, 98166 Messina, Italy
2
Department of Mathematics, Computer Sciences, Physics and Earth Sciences, University of Messina, V.le F. Stagno d’Alcontres 31, 98166 Messina, Italy
3
Department of Basic Medical Sciences for Radiation Damages, Molecular and Cellular Radiation Biology Group, NIRS/QST, Chiba 263-8555, Japan
4
Institute of Polar Sciences, National Research Council (CNR-ISP), Spianata San Raineri 86, 98122 Messina, Italy
5
Department of Theoretical and Applied Sciences, University of Insubria, Via J.H. Dunant, 21100 Varese, Italy
6
Institute of Aerospace Medicine, Radiation Biology Department, German Aerospace Center, Aerospace Microbiology, DLR, Linder Höhe, D-51147 Cologne/Köln, Germany
7
Natural Sciences Department, University of Applied Sciences Bonn-Rhein-Sieg (BRSU), D-53359 Rheinbach, Germany
*
Author to whom correspondence should be addressed.
Life 2020, 10(11), 264; https://doi.org/10.3390/life10110264
Received: 13 October 2020 / Revised: 27 October 2020 / Accepted: 28 October 2020 / Published: 30 October 2020
(This article belongs to the Special Issue Frontiers of Astrobiology)
Extremophiles are optimal models in experimentally addressing questions about the effects of cosmic radiation on biological systems. The resistance to high charge energy (HZE) particles, and helium (He) ions and iron (Fe) ions (LET at 2.2 and 200 keV/µm, respectively, until 1000 Gy), of spores from two thermophiles, Bacillushorneckiae SBP3 and Bacilluslicheniformis T14, and two psychrotolerants, Bacillus sp. A34 and A43, was investigated. Spores survived He irradiation better, whereas they were more sensitive to Fe irradiation (until 500 Gy), with spores from thermophiles being more resistant to irradiations than psychrotolerants. The survived spores showed different germination kinetics, depending on the type/dose of irradiation and the germinant used. After exposure to He 1000 Gy, D-glucose increased the lag time of thermophilic spores and induced germination of psychrotolerants, whereas L-alanine and L-valine increased the germination efficiency, except alanine for A43. FTIR spectra showed important modifications to the structural components of spores after Fe irradiation at 250 Gy, which could explain the block in spore germination, whereas minor changes were observed after He radiation that could be related to the increased permeability of the inner membranes and alterations of receptor complex structures. Our results give new insights on HZE resistance of extremophiles that are useful in different contexts, including astrobiology. View Full-Text
Keywords: Bacillus; extremophiles; heavy ion particle (HZE) radiations; nutrient germinants; spore germination; spore resistance Bacillus; extremophiles; heavy ion particle (HZE) radiations; nutrient germinants; spore germination; spore resistance
Show Figures

Graphical abstract

MDPI and ACS Style

Zammuto, V.; Rizzo, M.G.; De Plano, L.M.; Franco, D.; Guglielmino, S.; Caccamo, M.T.; Magazù, S.; Fujimori, A.; Giudice, A.L.; Guglielmin, M.; McAlpin, K.R.; Moeller, R.; Gugliandolo, C. Effects of Heavy Ion Particle Irradiation on Spore Germination of Bacillus spp. from Extremely Hot and Cold Environments. Life 2020, 10, 264.

AMA Style

Zammuto V, Rizzo MG, De Plano LM, Franco D, Guglielmino S, Caccamo MT, Magazù S, Fujimori A, Giudice AL, Guglielmin M, McAlpin KR, Moeller R, Gugliandolo C. Effects of Heavy Ion Particle Irradiation on Spore Germination of Bacillus spp. from Extremely Hot and Cold Environments. Life. 2020; 10(11):264.

Chicago/Turabian Style

Zammuto, Vincenzo; Rizzo, Maria G.; De Plano, Laura M.; Franco, Domenico; Guglielmino, Salvatore; Caccamo, Maria T.; Magazù, Salvatore; Fujimori, Akira; Giudice, Angelina L.; Guglielmin, Mauro; McAlpin, Kevin R.; Moeller, Ralf; Gugliandolo, Concetta. 2020. "Effects of Heavy Ion Particle Irradiation on Spore Germination of Bacillus spp. from Extremely Hot and Cold Environments" Life 10, no. 11: 264.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop