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The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol

1
IRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, Italy
2
Department of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, Italy
*
Author to whom correspondence should be addressed.
These authors equally contributed to this work as first authors.
Life 2020, 10(10), 227; https://doi.org/10.3390/life10100227
Received: 27 August 2020 / Revised: 29 September 2020 / Accepted: 30 September 2020 / Published: 1 October 2020
(This article belongs to the Section Pharmaceutical Science)
More than 120 cannabinoids were isolated from Cannabis sativa. In particular, Cannabidiol (CBD) and Cannabigerol (CBG) represent the two most studied non-psychoactive cannabinoids. However, CBG is less studied and less data are available on its biological properties and influence on synaptic transmission. On the contrary, CBD is already known to modulate brain excitatory glutamate, inhibitory γ-aminobutyric acid (GABA) and dopamine neurotransmission. In this study, using Next-Generation Sequencing (NGS) technology, we evaluated how CBG (1 or 5 µM) and CBD (1 or 5 µM) influence the transcriptome of the main neurotransmission pathways in NSC-34 motor neuron-like cells. At first, we evaluated that CBG and CBD were not cytotoxic and decreased the expression of pro-apoptotic genes. CBG and CBD are able to influence the expression of the genes involved in glutamate, GABA and dopamine signaling. Interestingly, the transcriptional changes induced by CBG were similar compared to CBD. View Full-Text
Keywords: Cannabidiol; Cannabigerol; Glutamatergic synapses; GABAergic synapses; motor neuron-like cells; transcriptomic analysis Cannabidiol; Cannabigerol; Glutamatergic synapses; GABAergic synapses; motor neuron-like cells; transcriptomic analysis
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MDPI and ACS Style

Gugliandolo, A.; Silvestro, S.; Chiricosta, L.; Pollastro, F.; Bramanti, P.; Mazzon, E. The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol. Life 2020, 10, 227. https://doi.org/10.3390/life10100227

AMA Style

Gugliandolo A, Silvestro S, Chiricosta L, Pollastro F, Bramanti P, Mazzon E. The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol. Life. 2020; 10(10):227. https://doi.org/10.3390/life10100227

Chicago/Turabian Style

Gugliandolo, Agnese, Serena Silvestro, Luigi Chiricosta, Federica Pollastro, Placido Bramanti, and Emanuela Mazzon. 2020. "The Transcriptomic Analysis of NSC-34 Motor Neuron-Like Cells Reveals That Cannabigerol Influences Synaptic Pathways: A Comparative Study with Cannabidiol" Life 10, no. 10: 227. https://doi.org/10.3390/life10100227

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