Next Article in Journal
Real-Time Observation of Fibrous Zeolites Reactivity in Contact with Simulated Lung Fluids (SLFs) Obtained by Atomic Force Microscope (AFM)
Previous Article in Journal
Anaerobic Bioreduction of Jarosites and Biofilm Formation by a Natural Microbial Consortium
Article Menu

Export Article

Open AccessArticle
Minerals 2019, 9(2), 82;

Responses of Acidithiobacillus thiooxidans A01 to Individual and Joint Nickel (Ni2+) and Ferric (Fe3+)

School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China
Key Laboratory of Biometallurgy of Ministry of Education, Changsha 410083, China
Author to whom correspondence should be addressed.
Received: 15 November 2018 / Revised: 2 January 2019 / Accepted: 10 January 2019 / Published: 29 January 2019
Full-Text   |   PDF [2337 KB, uploaded 14 February 2019]   |  
  |   Review Reports


Acidithiobacillus thiooxidans A01 is widely used in bioleaching processes and commonly thrives in most metal-rich environments. However, interactions between different heavy metals remain obscure. In this study, we elaborated the effect of ferric iron on the growth and gene expression of At. thiooxidans A01 under the stress of nickel. The results showed that 600 mM Ni2+ completely inhibited the growth and sulfur metabolism of At. thiooxidans A01. However, trace amounts of Fe3+ (0.5 mM) facilitated the growth of At. thiooxidans A01 in the presence of 600 mM Ni2+. With the addition of 5 mM Fe3+, the maximum cell density reached 1.84 × 108 cell/mL, and pH value was 0.95. In addition, metal resistance-related and sulfur metabolism genes were significantly up regulated with extra ferric iron. Taking the whole process into account, the promoting effect of Fe3+ addition can be attributed to the following: (1) alleviation of the effects of Ni2+ toxicity and restoring the growth of At. thiooxidans A01, (2) a choice of multiple pathways to export nickel ion and producing precursor of chelators of heavy metals. This can suggest that microorganisms may widely exhibit metabolic activity in iron-rich environments with heavy metals. Our study will facilitate the technique development for the processing of ore bodies with highly challenging ore compositions. View Full-Text
Keywords: Acidithiobacillus thiooxidans A01; nickel resistance; ferric iron; bioleaching Acidithiobacillus thiooxidans A01; nickel resistance; ferric iron; bioleaching

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Chen, A.; Hao, X.; Xiao, Y.; Zou, K.; Liu, H.; Liu, X.; Yin, H.; Qiu, G.; Liang, Y. Responses of Acidithiobacillus thiooxidans A01 to Individual and Joint Nickel (Ni2+) and Ferric (Fe3+). Minerals 2019, 9, 82.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Minerals EISSN 2075-163X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top