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Minerals 2015, 5(2), 335-345; https://doi.org/10.3390/min5020335

Use of Iron Powder to Obtain High Yields of Leptothrix Sheaths in Culture

1
Core Research for Evolutionary Science and Technology (CREST), Japan Science and Technology Agency (JST), Okayama 700-8530, Japan
2
Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Catherine W. Skinner
Received: 27 February 2015 / Accepted: 1 June 2015 / Published: 4 June 2015
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Abstract

The Leptothrix species, Fe-oxidizing bacteria, produce an extracellular, microtubular sheath with a complicated organic–inorganic hybrid nature. We have discovered diverse industrial functions for this material, e.g., electrode material for Li-ion batteries, catalyst enhancers, pigments, plant growth promoters, and plant protectants. To consistently obtain material with the qualitative and quantitative stability needed for industrial applications, we focused on developing an optimum culture system for sheath synthesis by the Leptothrix sp. strain OUMS1. Although we have used Fe plates as an Fe source in the liquid silicon-glucose-peptone medium (SGP), the plates do not yield a consistent quality or precise mass, and formation of Fe-encrusted sheath is restricted to a surface of the plates, which limits harvest yield. In this study, to obtain a high yield of sheaths, we cultured OUMS1 in SGP supplemented with Fe powders. The addition of Fe powders to the medium (up to 14.0 g/L) did not adversely influence growth of OUMS1. The final yield of sheaths was about 10-fold greater than in the Fe plate culture. The sheaths also maintained a microtubular form and crystalline texture similar to those produced on Fe plates in SGP. The results proved the usefulness of Fe powder for consistently high yields of Fe-encrusted sheaths of stable quality. View Full-Text
Keywords: Leptothrix sp.; high yield of Fe-encrusted sheaths; artificial medium with Fe powder; sheaths for industrial use Leptothrix sp.; high yield of Fe-encrusted sheaths; artificial medium with Fe powder; sheaths for industrial use
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Suzuki, T.; Kunoh, T.; Nakatsuka, D.; Hashimoto, H.; Tamura, K.; Kunoh, H.; Takada, J. Use of Iron Powder to Obtain High Yields of Leptothrix Sheaths in Culture. Minerals 2015, 5, 335-345.

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