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Open AccessArticle

Exploration and Modulation of Antibody Fragment Biophysical Properties by Replacing the Framework Region Sequences

1
INRAE, ISP, Université de Tours, F-37000 Tours, France
2
IRCM, Institut de Recherche en Cancérologie de Montpellier, INSERM, U1194, Université Montpellier, ICM Institut Régional du Cancer, 34090 Montpellier, France
3
Laboratório de Imunoquímica, Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba 81530, PR, Brazil
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Antibodies 2020, 9(2), 9; https://doi.org/10.3390/antib9020009
Received: 18 March 2020 / Revised: 12 April 2020 / Accepted: 13 April 2020 / Published: 15 April 2020
In order to increase the successful development of recombinant antibodies and fragments, it seems fundamental to enhance their expression and/or biophysical properties, such as the thermal, chemical, and pH stabilities. In this study, we employed a method bases on replacing the antibody framework region sequences, in order to promote more particularly single-chain Fragment variable (scFv) product quality. We provide evidence that mutations of the VH- C-C′ loop might significantly improve the prokaryote production of well-folded and functional fragments with a production yield multiplied by 27 times. Additional mutations are accountable for an increase in the thermal (+19.6 °C) and chemical (+1.9 M) stabilities have also been identified. Furthermore, the hereby-produced fragments have shown to remain stable at a pH of 2.0, which avoids molecule functional and structural impairments during the purification process. Lastly, this study provides relevant information to the understanding of the relationship between the antibodies amino acid sequences and their respective biophysical properties. View Full-Text
Keywords: engineering; framework regions; Protein L (PpL); single-chain Fragment variable (scFv); stabilities; production engineering; framework regions; Protein L (PpL); single-chain Fragment variable (scFv); stabilities; production
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MDPI and ACS Style

Cnudde, T.; Lakhrif, Z.; Bourgoin, J.; Boursin, F.; Horiot, C.; Henriquet, C.; di Tommaso, A.; Juste, M.O.; Jiacomini, I.G.; Dimier-Poisson, I.; Pugnière, M.; Mévélec, M.-N.; Aubrey, N. Exploration and Modulation of Antibody Fragment Biophysical Properties by Replacing the Framework Region Sequences. Antibodies 2020, 9, 9. https://doi.org/10.3390/antib9020009

AMA Style

Cnudde T, Lakhrif Z, Bourgoin J, Boursin F, Horiot C, Henriquet C, di Tommaso A, Juste MO, Jiacomini IG, Dimier-Poisson I, Pugnière M, Mévélec M-N, Aubrey N. Exploration and Modulation of Antibody Fragment Biophysical Properties by Replacing the Framework Region Sequences. Antibodies. 2020; 9(2):9. https://doi.org/10.3390/antib9020009

Chicago/Turabian Style

Cnudde, Thomas; Lakhrif, Zineb; Bourgoin, Justine; Boursin, Fanny; Horiot, Catherine; Henriquet, Corinne; di Tommaso, Anne; Juste, Matthieu O.; Jiacomini, Isabella G.; Dimier-Poisson, Isabelle; Pugnière, Martine; Mévélec, Marie-Nöelle; Aubrey, Nicolas. 2020. "Exploration and Modulation of Antibody Fragment Biophysical Properties by Replacing the Framework Region Sequences" Antibodies 9, no. 2: 9. https://doi.org/10.3390/antib9020009

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