Next Article in Journal
Back-to-Germline (B2G) Procedure for Antibody Devolution
Previous Article in Journal
Design and Production of Bispecific Antibodies
Previous Article in Special Issue
Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning
Open AccessReview

Phage Display Libraries for Antibody Therapeutic Discovery and Development

1
GlobalBio, Inc., 320, Cambridge, MA 02138, USA
2
UDIBI, ENCB, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala S/N, Colonia Casco de Santo Tomas, Delegación Miguel Hidalgo, Ciudad de Mexico 11340, Mexico
3
CONACyT-UDIBI, ENCB, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala S/N, Colonia Casco de Santo Tomas, Delegación Miguel Hidalgo, Ciudad de Mexico 11340, Mexico
*
Author to whom correspondence should be addressed.
Antibodies 2019, 8(3), 44; https://doi.org/10.3390/antib8030044
Received: 24 June 2019 / Revised: 14 August 2019 / Accepted: 15 August 2019 / Published: 23 August 2019
(This article belongs to the Special Issue Antibody Phage Display)
Phage display technology has played a key role in the remarkable progress of discovering and optimizing antibodies for diverse applications, particularly antibody-based drugs. This technology was initially developed by George Smith in the mid-1980s and applied by John McCafferty and Gregory Winter to antibody engineering at the beginning of 1990s. Here, we compare nine phage display antibody libraries published in the last decade, which represent the state of the art in the discovery and development of therapeutic antibodies using phage display. We first discuss the quality of the libraries and the diverse types of antibody repertoires used as substrates to build the libraries, i.e., naïve, synthetic, and semisynthetic. Second, we review the performance of the libraries in terms of the number of positive clones per panning, hit rate, affinity, and developability of the selected antibodies. Finally, we highlight current opportunities and challenges pertaining to phage display platforms and related display technologies. View Full-Text
Keywords: naive repertoires; synthetic repertoires; antibody library design; developability; hit rate; affinity naive repertoires; synthetic repertoires; antibody library design; developability; hit rate; affinity
Show Figures

Figure 1

MDPI and ACS Style

Almagro, J.C.; Pedraza-Escalona, M.; Arrieta, H.I.; Pérez-Tapia, S.M. Phage Display Libraries for Antibody Therapeutic Discovery and Development. Antibodies 2019, 8, 44.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop