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Review

Enzyme-Based Labeling Strategies for Antibody–Drug Conjugates and Antibody Mimetics

Cellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld, Germany
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Author to whom correspondence should be addressed.
Antibodies 2018, 7(1), 4; https://doi.org/10.3390/antib7010004
Received: 1 November 2017 / Revised: 15 December 2017 / Accepted: 18 December 2017 / Published: 4 January 2018
(This article belongs to the Special Issue Therapeutic Antibodies)
Strategies for site-specific modification of proteins have increased in number, complexity, and specificity over the last years. Such modifications hold the promise to broaden the use of existing biopharmaceuticals or to tailor novel proteins for therapeutic or diagnostic applications. The recent quest for next-generation antibody–drug conjugates (ADCs) sparked research into techniques with site selectivity. While purely chemical approaches often impede control of dosage or locus of derivatization, naturally occurring enzymes and proteins bear the ability of co- or post-translational protein modifications at particular residues, thus enabling unique coupling reactions or protein fusions. This review provides a general overview and focuses on chemo-enzymatic methods including enzymes such as formylglycine-generating enzyme, sortase, and transglutaminase. Applications for the conjugation of antibodies and antibody mimetics are reported. View Full-Text
Keywords: chemo-enzymatic labeling; armed antibody; antibody coupling; antibody conjugation chemo-enzymatic labeling; armed antibody; antibody coupling; antibody conjugation
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MDPI and ACS Style

Falck, G.; Müller, K.M. Enzyme-Based Labeling Strategies for Antibody–Drug Conjugates and Antibody Mimetics. Antibodies 2018, 7, 4. https://doi.org/10.3390/antib7010004

AMA Style

Falck G, Müller KM. Enzyme-Based Labeling Strategies for Antibody–Drug Conjugates and Antibody Mimetics. Antibodies. 2018; 7(1):4. https://doi.org/10.3390/antib7010004

Chicago/Turabian Style

Falck, Georg, and Kristian M. Müller. 2018. "Enzyme-Based Labeling Strategies for Antibody–Drug Conjugates and Antibody Mimetics" Antibodies 7, no. 1: 4. https://doi.org/10.3390/antib7010004

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