Next Article in Journal
Pharmacokinetic and Pharmacodynamic Considerations for the Use of Monoclonal Antibodies in the Treatment of Bacterial Infections
Next Article in Special Issue
Therapeutic Antibody-Like Immunoconjugates against Tissue Factor with the Potential to Treat Angiogenesis-Dependent as Well as Macrophage-Associated Human Diseases
Previous Article in Journal
Tumor-Directed Blockade of CD47 with Bispecific Antibodies Induces Adaptive Antitumor Immunity
Previous Article in Special Issue
Antagonist Anti-CD28 Therapeutics for the Treatment of Autoimmune Disorders
Article Menu

Export Article

Open AccessReview
Antibodies 2018, 7(1), 4; https://doi.org/10.3390/antib7010004

Enzyme-Based Labeling Strategies for Antibody–Drug Conjugates and Antibody Mimetics

Cellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld, Germany
*
Author to whom correspondence should be addressed.
Received: 1 November 2017 / Revised: 15 December 2017 / Accepted: 18 December 2017 / Published: 4 January 2018
(This article belongs to the Special Issue Therapeutic Antibodies)
Full-Text   |   PDF [3026 KB, uploaded 4 January 2018]   |  

Abstract

Strategies for site-specific modification of proteins have increased in number, complexity, and specificity over the last years. Such modifications hold the promise to broaden the use of existing biopharmaceuticals or to tailor novel proteins for therapeutic or diagnostic applications. The recent quest for next-generation antibody–drug conjugates (ADCs) sparked research into techniques with site selectivity. While purely chemical approaches often impede control of dosage or locus of derivatization, naturally occurring enzymes and proteins bear the ability of co- or post-translational protein modifications at particular residues, thus enabling unique coupling reactions or protein fusions. This review provides a general overview and focuses on chemo-enzymatic methods including enzymes such as formylglycine-generating enzyme, sortase, and transglutaminase. Applications for the conjugation of antibodies and antibody mimetics are reported. View Full-Text
Keywords: chemo-enzymatic labeling; armed antibody; antibody coupling; antibody conjugation chemo-enzymatic labeling; armed antibody; antibody coupling; antibody conjugation
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Falck, G.; Müller, K.M. Enzyme-Based Labeling Strategies for Antibody–Drug Conjugates and Antibody Mimetics. Antibodies 2018, 7, 4.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Antibodies EISSN 2073-4468 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top