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Antibodies 2017, 6(4), 22; https://doi.org/10.3390/antib6040022

Generation and Performance of R132H Mutant IDH1 Rabbit Monoclonal Antibody

MilliporeSigma, 6600 Sierra College Blvd, Rocklin, CA 95677, USA
These authors contributed equally to this paper.
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Author to whom correspondence should be addressed.
Received: 31 October 2017 / Revised: 21 November 2017 / Accepted: 22 November 2017 / Published: 1 December 2017
(This article belongs to the Special Issue Monoclonal Antibodies)
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Abstract

Isocitrate dehydrogenase 1 (IDH1) gene mutations have been observed in a majority of diffuse astrocytomas, oligodendrogliomas, and secondary glioblastomas, and the mutant IDH1 R132H is detectable in most of these lesions. By specifically targeting the R132H mutation through B-cell cloning, a novel rabbit monoclonal antibody, MRQ-67, was produced that can recognize mutant IDH1 R132H and does not react with the wild type protein as demonstrated by Enzyme-linked immunosorbent assay (ELISA) and Western blotting. Through immunohistochemistry, the antibody is able to highlight neoplastic cells in glioma tissue specimens, and can be used as a tool in glioma subtyping. Immunohistochemistry (IHC) detection of IDH1 mutant protein may also be used to visualize single infiltrating tumor cells in surrounding brain tissue with an otherwise normal appearance. View Full-Text
Keywords: IDH1; R132H; novel rabbit monoclonal antibody; B-cell cloning; immunohistochemistry IDH1; R132H; novel rabbit monoclonal antibody; B-cell cloning; immunohistochemistry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Rashidian, J.; Copaciu, R.; Su, Q.; Merritt, B.; Johnson, C.; Yahyabeik, A.; French, E.; Cummings, K. Generation and Performance of R132H Mutant IDH1 Rabbit Monoclonal Antibody. Antibodies 2017, 6, 22.

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