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Open AccessReview

Generating Recombinant Antibodies to Membrane Proteins through Phage Display

1
Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607-7060, USA
2
AxioMx Inc., a subsidiary of Abcam Plc, Branford, CT 06405, USA
*
Author to whom correspondence should be addressed.
Current address: Meso Scale Diagnostics LLC, Rockville, MD 20850, USA
Academic Editor: Rui Gong
Antibodies 2016, 5(2), 11; https://doi.org/10.3390/antib5020011
Received: 25 March 2016 / Revised: 21 April 2016 / Accepted: 21 April 2016 / Published: 2 May 2016
One of the most important classes of proteins in terms of drug targets is cell surface membrane proteins, and yet it is a challenging set of proteins for generating high-quality affinity reagents. In this review, we focus on the use of phage libraries, which display antibody fragments, for generating recombinant antibodies to membrane proteins. Such affinity reagents generally have high specificity and affinity for their targets. They have been used for cell staining, for promoting protein crystallization to solve three-dimensional structures, for diagnostics, and for treating diseases as therapeutics. We cover publications on this topic from the past 10 years, with a focus on the various formats of membrane proteins for affinity selection and the diverse affinity selection strategies used. Lastly, we discuss the challenges faced in this field and provide possible directions for future efforts. View Full-Text
Keywords: affinity selection; fragments of antigen binding (Fabs); G-protein coupled receptors (GPCRs); membrane proteins; nanodiscs; phage-display; recombinant antibodies; single-domain antibodies; transfection; virus-like particles (VLPs) affinity selection; fragments of antigen binding (Fabs); G-protein coupled receptors (GPCRs); membrane proteins; nanodiscs; phage-display; recombinant antibodies; single-domain antibodies; transfection; virus-like particles (VLPs)
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MDPI and ACS Style

Huang, R.; Kiss, M.M.; Batonick, M.; Weiner, M.P.; Kay, B.K. Generating Recombinant Antibodies to Membrane Proteins through Phage Display. Antibodies 2016, 5, 11.

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