Soil Amendments Alter Ammonia-Oxidizing Archaea and Bacteria Communities in Rain-Fed Maize Field in Semi-Arid Loess Plateau
Abstract
:1. Introduction
2. Materials and Methods
2.1. Description of the Experimental Site
2.2. Experimental Design and Soil Sampling
2.3. Soil Chemical Properties
2.4. DNA Isolation and Quantitative PCR Analysis
2.5. High-Throughput Sequencing and Bioinformatic Analysis
2.6. Data Analysis
2.7. Network, SEM, and Random Forest Analysis
3. Results
3.1. Soil Physicochemical Properties and Aboveground Biomass
3.2. Abundance and Diversity of Ammonia Oxidizers with Its Association to Soil Physiochemical Properties
3.3. The Compositions of Ammonia Oxidizers
3.4. The Co-Occurrence Network of Ammonia Oxidizers and Its Association of Major Modules with Environmental Variables
3.5. Soil Properties and Ammonia Oxidizers Regulated the PNA and Crop Productivity (Yield)
4. Discussion
4.1. The Impact of Fertilizer Treatments in Calcareous Soils on the Abundance and Diversity of AOA and AOB
4.2. The Changes in the Co-Occurrence Network of AOA and AOB Communities among Soil Amendments
4.3. Relative Contributions of AOA and AOB Community to Soil Nitrification
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Soil Depth (cm) | Bulk Density (mg/m3) | pH | Total Nitrogen (g/kg) | Total Phosphorus (g/kg) | Organic C (g/kg) |
---|---|---|---|---|---|
0–5 | 1.19 | 8.33 | 1.05 | 0.82 | 9.91 |
5–10 | 1.22 | 8.32 | 1.05 | 0.74 | 8.96 |
10–30 | 1.28 | 8.37 | 0.94 | 0.7 | 8.89 |
Amendment | Organic Carbon | N | P | K | Ca | Mg |
---|---|---|---|---|---|---|
Corn stalk | 47.5 | 0.74 | 0.38 | 0.45 | 0.55 | 0.74 |
Cow manure | 39.9 | 2.20 | 1.70 | 1.90 | 2.70 | 0.50 |
Soil | pH | TN | SOC | NO3−-N | NH4+-N | AP |
---|---|---|---|---|---|---|
Amendment | (g/kg) | (g/kg) | (mg/kg) | (mg/kg) | (mg/kg) | |
NA | 8.66 ± 0.03 ab | 0.85 ± 0.01 b | 7.48 ± 0.18 c | 17.84 ± 1.04 c | 15.33 ± 1.63 a | 9.73 ± 1.41 c |
CF | 8.32 ± 0.07 c | 0.93 ± 0.03 a | 7.93 ± 0.07 c | 30.81 ± 2.78 a | 16.07 ± 1.48 a | 16.70 ± 1.57 ab |
SC | 8.44 ± 0.08 bc | 0.93 ± 0.02 a | 8.84 ± 0.20 b | 28.40 ± 3.57 ab | 14.87 ± 2.22 a | 18.32 ± 1.69 ab |
SM | 8.45 ± 0.05 bc | 0.98 ± 0.03 a | 8.81 ± 0.33 b | 25.40 ± 3.41 abc | 15.81 ± 1.36 a | 19.81 ± 0.72 a |
MS | 8.54 ± 0.06 b | 0.99 ± 0.002 a | 9.82 ± 0.19 a | 21.93 ± 1.81 bc | 16.53 ± 2.80 a | 15.14 ± 0.97 b |
p-value | 0.013 | 0.001 | 0.036 | 0.024 | 0.107 | 0.002 |
Treatment | Observed Species | OTUs | Chao1 | Shannon | Simpson | Goods Coverage |
---|---|---|---|---|---|---|
NA | 4865.1 ± 49.6 a | 518.1 ± 10.1 a | 629 ± 36.8 a | 0.9 ± 0.0 a | 2.9 ± 0.04 a | 0.21 ± 0.01 a |
CF | 4297.9 ± 74.6 a | 524.3 ± 50.4 a | 641 ± 74.3 a | 0.9 ± 0.0 a | 3.2 ± 0.12 a | 0.17 ± 0.02 a |
SC | 5331.2 ± 20.1 a | 557.2 ± 22.5 a | 643 ± 43.1 a | 0.9 ± 0.0 a | 2.98 ± 0.11 a | 0.12 ± 0.02 a |
SM | 4434.3 ± 10.5 a | 551 ± 13.51 a | 631 ± 49.7 a | 0.9 ± 0.0 a | 3.04 ± 0.16 a | 0.19 ± 0.02 a |
MS | 4329.4 ± 10.9 a | 521 ± 50.4 a | 618 a ± 38.2 a | 0.9 ± 0.1 a | 3.07 ± 0.33 a | 0.18 ± 0.01 a |
p-value | <0.482 | 0.657 | 0.969 | 0.296 | 0.716 | 0.331 |
Treatment | Observed Species | OTUs | Chao1 | Shannon | Simpson | Goods Coverage |
---|---|---|---|---|---|---|
NA | 4111.7 ± 83.2 a | 1296.3 ± 147.6 a | 1843 ± 27.3 a | 4.1 ± 0.25 a | 0.03 ± 0.01 ab | 0.98 ± 0.01 a |
CF | 3980.7 ± 56.7 a | 1989.6 ± 81.5 b | 2691 ± 23.1 b | 5.0 ± 0.11 b | 0.02 ± 0.01 a | 0.99 ± 0.02 a |
SC | 4405.5 ± 77.7 a | 2104 ± 33.6 b | 3141.7 ± 51.6 b | 4.8 ± 0.21 b | 0.02 ± 0.01 a | 0.98 ± 0.04 a |
SM | 5039.5 ± 57.8 a | 1560 ± 30.7 ab | 2129.3 ± 35.6 a | 4.5 ± 0.56 ab | 0.03 ± 0.01 ab | 0.97 ± 0.01 a |
MS | 2153.9 ± 30.3 a | 1359 ± 96.2 a | 1877 ± 17.1 b | 4.4 ± 0.01 ab | 0.04 ± 0.01 b | 0.98 ± 0.02 a |
p-value | <0.471 | 0.003 | 0.001 | 0.049 | 0.13 | 0.205 |
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Fudjoe, S.K.; Li, L.; Jiang, Y.; Karikari, B.; Xie, J.; Wang, L.; Anwar, S.; Wang, J. Soil Amendments Alter Ammonia-Oxidizing Archaea and Bacteria Communities in Rain-Fed Maize Field in Semi-Arid Loess Plateau. Land 2021, 10, 1039. https://doi.org/10.3390/land10101039
Fudjoe SK, Li L, Jiang Y, Karikari B, Xie J, Wang L, Anwar S, Wang J. Soil Amendments Alter Ammonia-Oxidizing Archaea and Bacteria Communities in Rain-Fed Maize Field in Semi-Arid Loess Plateau. Land. 2021; 10(10):1039. https://doi.org/10.3390/land10101039
Chicago/Turabian StyleFudjoe, Setor Kwami, Lingling Li, Yuji Jiang, Benjamin Karikari, Junhong Xie, Linlin Wang, Sumera Anwar, and Jinbin Wang. 2021. "Soil Amendments Alter Ammonia-Oxidizing Archaea and Bacteria Communities in Rain-Fed Maize Field in Semi-Arid Loess Plateau" Land 10, no. 10: 1039. https://doi.org/10.3390/land10101039