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Water 2017, 9(9), 681;

Pseudomonas aeruginosa Psl Exopolysaccharide Interacts with the Antimicrobial Peptide LG21

Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore
School of Biological Sciences, Nanyang Technological University, Singapore 639798, Singapore
Advanced Environmental Biotechnology Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore
Interdisciplinary Graduate School, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
Departments of Genetics, Louisiana State University Health Sciences Center, Louisiana Cancer Research Center, 1700 Tulane Ave., New Orleans, LA 70112, USA
State Key Laboratory of Microbial Resources in Institute of Microbiology, Chinese Academy of Sciences (IMCAS), Beijing 100101, China
Author to whom correspondence should be addressed.
Received: 9 June 2017 / Revised: 30 August 2017 / Accepted: 4 September 2017 / Published: 16 September 2017
(This article belongs to the Special Issue Antimicrobial Resistance in Environmental Waters)
PDF [3849 KB, uploaded 16 September 2017]


Biofilm formation by opportunistic pathogens serves as one of the major causes of chronic and persistent infections. Bacterial cells in the biofilms are embedded in their self-generated protective extracellular polymeric substances (EPS), which include exopolysaccharides, large adhesin proteins and extracellular DNA. In this study, we identified an antimicrobial peptide (AMP) LG21 that is able to interact specifically with the Psl exopolysaccharide of Pseudomonas aeruginosa, thus it can be used as a diagnostic tool for P. aeruginosa biofilms. Molecular dynamics simulation analysis showed that residues numbered from 15 to 21 (WKRKRFG) in LG21 are involved in interacting with Psl. Our study indicates that host immune systems might detect and interact with microbial biofilms through AMPs. Engineering biofilm EPS-targeting AMPs might provide novel strategies for biofilm detection and treatment. View Full-Text
Keywords: Pseudomonas aeruginosa; Psl; exopolysaccharide; antimicrobial peptide (AMP); biofilm; EPS Pseudomonas aeruginosa; Psl; exopolysaccharide; antimicrobial peptide (AMP); biofilm; EPS

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Chin, J.S.F.; Sinha, S.; Nalaparaju, A.; Yam, J.K.H.; Qin, Z.; Ma, L.; Liang, Z.-X.; Lu, L.; Bhattacharjya, S.; Yang, L. Pseudomonas aeruginosa Psl Exopolysaccharide Interacts with the Antimicrobial Peptide LG21. Water 2017, 9, 681.

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