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Microbiological Confinement of Two Adjacent Water Wells in Lake Karla Basin, Greece

Department of Ichthyology & Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Volos 384 46, Greece
Author to whom correspondence should be addressed.
Academic Editor: Robin Slawson
Water 2015, 7(10), 5272-5283;
Received: 8 September 2015 / Revised: 21 September 2015 / Accepted: 26 September 2015 / Published: 30 September 2015
PDF [886 KB, uploaded 30 September 2015]


We analyzed the bacterial and archaeal community structure of two adjacent irrigation well waters of the Lake Karla Basin, Central Greece, in order to elucidate their connectivity or confinement by using 454 tag pyrosequencing of the 16S rRNA genes. Although considerable overlap was found at the phylum/high taxonomic level, and also at the operational taxonomic units (OTU) level, the dominant, and most likely active, prokaryotes represented by these OTUs were very different between the two wells. As expected, we found higher bacterial species richness compared to that of archaeal, and this renders Bacteria better for the study of connectivity or confinement of water wells. Some of the taxonomic groups found are amongst those found typically in the terrestrial subsurface and also those that have been recently described, enhancing the importance of the subsurface for expanding our knowledge on microbial diversity. The majority of the archaeal and several of the bacterial OTUs, including the most dominant ones in each well, were related to marine or saline environments, indicating the previously suggested persistence of fertilizer residuals in the basin’s soils. View Full-Text
Keywords: bacteria; archaea; terrestrial subsurface; water bacteria; archaea; terrestrial subsurface; water

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Kormas, K.A.; Meziti, A.; Papadimitriou, T. Microbiological Confinement of Two Adjacent Water Wells in Lake Karla Basin, Greece. Water 2015, 7, 5272-5283.

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