Effects of Copper Stress on Nitrogen Metabolism-Related Enzymes in Nymphoides peltata

Copper (Cu) pollution poses environmental and health risks. Owing to its adaptability and potential for water purification, Nymphoides peltata (N. peltata) is being considered for use in the remediation of Cu pollution. However, the feasibility of using N. peltata for the remediation of Cu-polluted water bodies has not yet been assessed. Here, the physiological response of N. peltata to Cu stress was determined. N. peltata samples were exposed to varying Cu concentrations (0.2, 0.4, 0.6 and 0.8 mg∙L⁻¹), and the activities of glutamine synthetase (GS), nitrate reductase (NR), nitrite reductase (NiR), ribulose-1,5-diphosphate carboxylase (Rubisco), and glycolate oxidase (GO) were measured together with the concentrations of photosynthetic pigments. The results revealed that under Cu stress, NR and GS activities significantly decreased, while NiR activity significantly increased. Exposure to 0.2 mg∙L⁻¹ Cu promoted chlorophyll synthesis and enhanced Rubisco and GO activities; in contrast, exposure to Cu concentrations above 0.4 mg∙L⁻¹ significantly inhibited the aforementioned parameters. These findings indicate that Cu stress, regardless of concentration, significantly affects nitrogen metabolism in N. peltata by decelerating nitrate reduction and impairing the ammonification process. Meanwhile, only high Cu concentrations significantly affected photosynthesis. N. peltata can survive low Cu stress by regulating its photosynthetic enzymes. Therefore, N. peltata has potential for the ecological restoration of water bodies polluted with low Cu concentrations.