Thermal microstructure profiling is an established technique for investigating turbulent mixing and stratification in lakes and oceans. However, it provides only quasi-instantaneous, 1-D snapshots. Other approaches to measuring these phenomena exist, but each has logistic and/or quality weaknesses. Hence, turbulent mixing and stratification processes remain greatly under-sampled. This paper contributes to addressing this problem by presenting a novel analysis of thermal microstructure profiles, focusing on their multi-scale stratification structure. Profiles taken in two small lakes using a Self-Contained Automated Micro-Profiler (SCAMP) were analysed. For each profile, buoyancy frequency (N), Thorpe scales (L
T), and the coefficient of vertical turbulent diffusivity (K
Z) were determined. To characterize the multi-scale stratification, profiles of d
2T/dz
2 at a spectrum of scales were calculated and the number of turning points in them counted. Plotting these counts against the scale gave pseudo-spectra, which were characterized by the index D of their power law regression lines. Scale-dependent correlations of D with N, L
T and K
Z were found, and suggest that this approach may be useful for providing alternative estimates of the efficiency of turbulent mixing and measures of longer-term averages of K
Z than current methods provide. Testing these potential uses will require comparison of field measurements of D with time-integrated K
Z values and numerical simulations.
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