Next Article in Journal
Biochar as an Eco-Friendly and Economical Adsorbent for the Removal of Colorants (Dyes) from Aqueous Environment: A Review
Previous Article in Journal
Optimization of Hydrologic Response Units (HRUs) Using Gridded Meteorological Data and Spatially Varying Parameters
Article

Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond

Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Department of Ecology, Institute of Hydrobiology, School of Life Science and Technology, Ministry of Education, Jinan University, West 601 Huangpu Avenue, Tianhe District, Guangzhou 510632, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Water 2020, 12(12), 3559; https://doi.org/10.3390/w12123559
Received: 21 November 2020 / Revised: 12 December 2020 / Accepted: 12 December 2020 / Published: 18 December 2020
(This article belongs to the Section Wastewater Treatment and Reuse)
Overloading of ammonia and nitrite nitrogen in aquaculture can result in toxicity to aquatic animals. In order to eliminate the hazardous substances, a highly efficient denitrifying bacterium, Bacillus sp. SC16, was identified in a fishery pond and isolated subsequently. The strain SC16 could remove nitrate up to 97%, ammonia up to 36.6%, and nitrite up to 99.99% when incubated with nitrate at an initial concentration of 306.9 mg·L−1 for 72 h, ammonia at 165.49 mg·L−1 for 48 h, and nitrite at 200 mg·L−1 for 24 h under aerobic conditions. The nitrite reductase gene was identified as the nirK gene. The transcriptional levels of the nirK gene in strain SC16 incubated with ammonia, nitrate, and nitrite showed similar expression patterns. When the strain SC16 was used to treat the aquaculture water, the concentration of ammonia decreased significantly, from 8.35 mg·L−1 to 4.56 mg·L−1, and there was almost no accumulation of nitrite by the end of experiment. Therefore, the results indicated that Bacillus sp. SC16 could be a promising candidate for aquaculture water treatment. View Full-Text
Keywords: Bacillus sp.; denitrification; nirK gene; transcriptional expression; aquaculture Bacillus sp.; denitrification; nirK gene; transcriptional expression; aquaculture
Show Figures

Graphical abstract

MDPI and ACS Style

Sang, C.-G.; Fu, Y.-W.; Guo, S.-Q.; Luo, J.-J.; Zhang, Q.-Z. Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond. Water 2020, 12, 3559. https://doi.org/10.3390/w12123559

AMA Style

Sang C-G, Fu Y-W, Guo S-Q, Luo J-J, Zhang Q-Z. Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond. Water. 2020; 12(12):3559. https://doi.org/10.3390/w12123559

Chicago/Turabian Style

Sang, Chi-Guo; Fu, Yao-Wu; Guo, Shu-Quan; Luo, Jia-Ju; Zhang, Qi-Zhong. 2020. "Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond" Water 12, no. 12: 3559. https://doi.org/10.3390/w12123559

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop