Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond
Abstract
:1. Introduction
2. Materials and Methods
2.1. Media
2.2. Isolation and Identification
2.3. Nitrogen Removal Performance
2.4. Enzyme Assay
2.5. Amplification of the Functional Genes
2.6. Quantitative Real-Time PCR (QRT-PCR) Assay
2.7. The Performance of Strain SC16 in Aquaculture Water
2.8. Analytical Methods
2.9. Statistical Analysis
3. Results
3.1. Isolation and Identification of Strain SC16
3.2. Evaluation of Nitrogen Removal Ability of Strain SC16
3.3. Assessment of Nitrite Removal Performance of SC16 under Different Nitrite Concentrations
3.4. Enzyme Assay
3.5. Amplification of the Denitrification Genes
3.6. Effects of Nitrogen Sources on the Expression Level of Nitrite Reductase
3.7. The Application of Strain SC16 in Aquaculture Water
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Time (h) | Nitrite Concentration (mg·L−1) | ||||||||
---|---|---|---|---|---|---|---|---|---|
0.1 | 2 | 50 | 100 | 200 | 250 | 300 | 500 | 1000 | |
0 | 0.091 ± 0.03 | 1.98 ± 0.088 | 49.52 ± 0.29 | 99.71 ± 0.19 | 199.76 ± 6.39 | 248.57 ± 3.29 | 297.25 ± 5.58 | 499.46 ± 17.26 | 992.96 ± 11.17 |
12 | 0.031 ± 0.05 | 0.043 ± 0.027 | 0.2 ± 0.15 | 0.71 ± 0.19 | 156.84 ± 4.35 | 200.48 ± 5.89 | 249.65 ± 9.19 | 438.24 ± 18.19 | 934.81 ± 14.07 |
24 | 0.02 ± 0.05 | 0.043 ± 0.058 | 0.1 ± 0.17 | 0.071 ± 0.17 | 1.29 ± 1.19 | 176.78 ± 1.58 | 246.89 ± 7.13 | 435.2 ± 16.52 | 933.62 ± 13.32 |
36 | 0.015 ± 0.02 | 0.04 ± 0.02 | 0.02 ± 0.01 | 0.07 ± 0.05 | 0.11 ± 0.18 | 138.76 ± 3.19 | 245.53 ± 6.73 | 434.34 ± 9.8 | 932.42 ± 12.3 |
48 | 0.01 ± 0.03 | 0.03 ± 0.02 | 0.015 ± 0.011 | 0.04 ± 0.02 | 0.039 ± 0.02 | 106.78 ± 2.79 | 240.89 ± 9.87 | 430.25 ± 11.23 | 932.23 ± 10.02 |
Removal a | 0.09 ± 0.03 | 1.97 ± 0.02 | 49.985 ± 0.011 | 99.96 ± 0.02 | 199.98 ± 0.02 | 143.22 ± 2.79 | 59.11 ± 9.87 | 69.75 ± 11.23 | 67.77 ± 10.02 |
Removal percentage (%) b | 90 ± 0.3 | 98.5 ± 0.01 | 99.97 ± 0.00 | 99.96 ± 0.00 | 99.99 ± 0.00 | 57.29 ± 0.01 | 19.7 ± 0.03 | 13.95 ± 0.02 | 6.78 ± 0.01 |
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Sang, C.-G.; Fu, Y.-W.; Guo, S.-Q.; Luo, J.-J.; Zhang, Q.-Z. Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond. Water 2020, 12, 3559. https://doi.org/10.3390/w12123559
Sang C-G, Fu Y-W, Guo S-Q, Luo J-J, Zhang Q-Z. Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond. Water. 2020; 12(12):3559. https://doi.org/10.3390/w12123559
Chicago/Turabian StyleSang, Chi-Guo, Yao-Wu Fu, Shu-Quan Guo, Jia-Ju Luo, and Qi-Zhong Zhang. 2020. "Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond" Water 12, no. 12: 3559. https://doi.org/10.3390/w12123559