Achieving Partial Nitrification via Intermittent Aeration in SBR and Short-Term Effects of Different C/N Ratios on Reactor Performance and Microbial Community Structure
Abstract
:1. Introduction
2. Materials and Methods
2.1. The SBR Setup and Operation Protocol
2.2. Synthetic Wastewater and Seed Sludge
2.3. Batch Tests
2.4. Analytical Methods
2.5. DNA Extraction, PCR Amplification, and High-Throughput Sequencing Analysis
3. Results and Discussion
3.1. Achievement of Partial Nitrification via Intermittent Aeration
3.2. Short-Term Effects of Different C/N Ratios on the Reactor Performance
3.3. Microbial Community Analysis
3.3.1. Changes of Microbial Community after Achieving Partial Nitrification
3.3.2. Shift of Microbial Community under Different C/N Ratios
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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C/N | Cycle | SAOB | SNOB |
---|---|---|---|
(mg N/(g MLSS·h)) | (mg N/(g MLSS·h)) | ||
4.64 | 29 | 5.25 | 3.48 |
113 | 3.89 | 0.96 | |
160 | 4.29 | 0.83 | |
3.87 | 196 | 5.51 | 1.01 |
2.32 | 238 | 6.29 | 1.04 |
0.77 | 281 | 2.04 | Almost zero |
Samples | Sobs | Shannon | Simpson | Ace | Chao | Coverage |
---|---|---|---|---|---|---|
S0 | 913 | 5.25 | 0.020 | 967.5 | 968.3 | 0.9968 |
S1 | 627 | 3.73 | 0.091 | 754.9 | 742.9 | 0.9953 |
S2 | 481 | 3.62 | 0.055 | 639.7 | 626.0 | 0.9955 |
S3 | 462 | 3.72 | 0.067 | 595.2 | 605.2 | 0.9961 |
S4 | 416 | 3.69 | 0.069 | 508.2 | 506.0 | 0.9969 |
Denitrifying Bacteria | S1 | S2 | S3 | S4 |
---|---|---|---|---|
Candidatus_Competibacter | 0.8% | 0.5% | 0.7% | 0.3% |
Dechloromonas | 1.0% | 10.3% | 2.7% | 0.9% |
Denitratisoma | 0.0% | 0.0% | 0.2% | 0.3% |
Dokdonella | 0.0% | 0.0% | 0.0% | 0.2% |
Hyphomicrobium | 0.2% | 0.4% | 0.4% | 0.6% |
Pseudomonas | 0.0% | 0.0% | 0.0% | 0.0% |
Rhodobacter | 0.3% | 1.3% | 2.9% | 3.1% |
Terrimonas | 0.1% | 0.0% | 0.1% | 0.1% |
Thauera | 0.1% | 0.0% | 0.0% | 0.0% |
Zoogloea | 1.8% | 0.3% | 0.1% | 0.0% |
total | 4.3% | 12.9% | 7.0% | 5.5% |
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Zhou, Z.; Qi, M.; Wang, H. Achieving Partial Nitrification via Intermittent Aeration in SBR and Short-Term Effects of Different C/N Ratios on Reactor Performance and Microbial Community Structure. Water 2020, 12, 3485. https://doi.org/10.3390/w12123485
Zhou Z, Qi M, Wang H. Achieving Partial Nitrification via Intermittent Aeration in SBR and Short-Term Effects of Different C/N Ratios on Reactor Performance and Microbial Community Structure. Water. 2020; 12(12):3485. https://doi.org/10.3390/w12123485
Chicago/Turabian StyleZhou, Zhengyao, Meixia Qi, and Hongping Wang. 2020. "Achieving Partial Nitrification via Intermittent Aeration in SBR and Short-Term Effects of Different C/N Ratios on Reactor Performance and Microbial Community Structure" Water 12, no. 12: 3485. https://doi.org/10.3390/w12123485