The ecological water regime in lake water bodies refers to the water levels that enable the fulfillment of the ecosystem’s multiple functions. Therefore, assessing the ecological water regime necessitates the consideration of hydrological, economic, social, and ecological factors. The present research is focused on the assessment of the ecological water level of four Mediterranean natural lake ecosystems, considering their morphological and biological features. Initially, suggestions on the ecological water regime of the studied lakes were made based on an analysis of the lakes’ morphometry. Further, the ecological and biological requirements of the present fish fauna and aquatic macrophytic vegetation were considered. For the latter, mapping was conducted by extensive sampling according to international standards, in order to assess macrophyte composition, abundance, and chorology, as well as species sensitivity to water level fluctuations. The above guided the proposals on the optimal water level regime that should be met by each lake regarding the macrophytic and fish communities’ sustainability, also taking into account the unique hydromorphological features of each lake. The differences in the outcoming results revealed that hydromorphological and biological approaches should be combined for assessing lakes’ ecological water regimes.
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