Next Article in Journal
Genome Sequence of the Freshwater Yangtze Finless Porpoise
Previous Article in Journal
Chromatin Architectural Changes during Cellular Senescence and Aging
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Genes 2018, 9(4), 212;

Transcriptomic Analysis of Flower Bud Differentiation in Magnolia sinostellata

College of Landscape and Architecture, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China
Xian Botanical Garden of Shaanxi Academy of Science, Xian 710061, China
Shenzhen Fairy Lake Botanical Garden, Shenzhen 518004, China
Author to whom correspondence should be addressed.
Received: 12 February 2018 / Revised: 8 April 2018 / Accepted: 11 April 2018 / Published: 16 April 2018
(This article belongs to the Section Plant Genetics and Genomics)
Full-Text   |   PDF [31469 KB, uploaded 3 May 2018]   |  


Magnolias are widely cultivated for their beautiful flowers, but despite their popularity, the molecular mechanisms regulating flower bud differentiation have not been elucidated. Here, we used paraffin sections and RNA-seq to study the process of flower bud differentiation in Magnolia sinostellata. Flower bud development occurred between 28 April and 30 May 2017 and was divided into five stages: undifferentiated, early flower bud differentiation, petal primordium differentiation, stamen primordium differentiation, and pistil primordium differentiation. A total of 52,441 expressed genes were identified, of which 11,592 were significantly differentially expressed in the five bud development stages. Of these, 82 genes were involved in the flowering. In addition, MADS-box and AP2 family genes play critical roles in the formation of flower organs and 20 differentially expressed genes associated with flower bud differentiation were identified in M. sinostellata. A qRT-PCR analysis verified that the MADS-box and AP2 family genes were expressed at high levels during flower bud differentiation. Consequently, this study provides a theoretical basis for the genetic regulation of flowering in M. sinostellata, which lays a foundation for further research into flowering genes and may facilitate the development of new cultivars. View Full-Text
Keywords: flower bud differentiation; flowering gene; RNA-seq; qRT-PCR; Magnolia sp. flower bud differentiation; flowering gene; RNA-seq; qRT-PCR; Magnolia sp.

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Fan, L.; Chen, M.; Dong, B.; Wang, N.; Yu, Q.; Wang, X.; Xuan, L.; Wang, Y.; Zhang, S.; Shen, Y. Transcriptomic Analysis of Flower Bud Differentiation in Magnolia sinostellata. Genes 2018, 9, 212.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top