Next Article in Journal
Fragmented Nuclear DNA Is the Predominant Genetic Material in Human Hair Shafts
Previous Article in Journal
The 5′-3′ Exoribonuclease XRN4 Regulates Auxin Response via the Degradation of Auxin Receptor Transcripts
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Genes 2018, 9(12), 639; https://doi.org/10.3390/genes9120639

De Novo Analysis Reveals Transcriptomic Responses in Eriobotrya japonica Fruits during Postharvest Cold Storage

1
Key Laboratory of Loquat Germplasm Innovation and Utilization (Putian University), Fujian Province University, Putian 351100, China
2
Fujian Provincial Key Laboratory of Ecology-toxicological Effects & Control for Emerging Contaminants, Putian University, Putian 351100, China
3
Faculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, Canada
4
Overseas Education College, Fujian Agriculture and Forestry University, Fuzhou 350002, China
5
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
*
Author to whom correspondence should be addressed.
Received: 21 October 2018 / Revised: 14 November 2018 / Accepted: 11 December 2018 / Published: 17 December 2018
(This article belongs to the Section Plant Genetics and Genomics)
  |  
PDF [7607 KB, uploaded 19 December 2018]
  |  

Abstract

Cold storage is the primary preservation method of postharvest loquat fruits. However, cold storage also results in many chilling injury physiological disorders called lignification, which decreases the quality and economic value of the fruits. Few studies to date have focused on the transcriptomic responses associated with lignification except lignin synthesis pathways. This study aimed to explore the changes of loquat transcriptome during long-term cold storage. Our results showed that the gene expression patterns were differed among the five stages. The differentially expressed genes (DEGs) in response to cold storage were more intense and complex in earlier stage. The membrane-related genes preferentially responded to low temperature and were followed by intracellular-located genes. The cold-induced pathways were mainly concerned with signal transduction and secondary metabolism (i.e., lignin, pectin, cellulose, terpenoid, carotenoid, steroid) in the first three stages and were chiefly related to primary metabolism in the later two stages, especially energy metabolism. Further investigation suggested that 503 protein kinases, 106 protein phosphatases, and 40 Ca2+ signal components were involved in the cold signal transduction of postharvest loquat fruits. We predicted a pathway including 649 encoding genes of 49 enzymes, which displayed the metabolisms of major sugars and polysaccharides in cold-stored loquat fruits. The coordinated expression patterns of these genes might contribute to the changes of saccharides in the pathway. These results provide new insight into the transcriptomic changes of postharvest loquat fruits in response to cold storage environment, which may be helpful for improving the postharvest life of loquat in the future. View Full-Text
Keywords: loquat fruit; transcriptomic responses; lignification; postharvest cold storage; RNA-seq; gene expression; signal transduction; sugars and polysaccharides metabolism loquat fruit; transcriptomic responses; lignification; postharvest cold storage; RNA-seq; gene expression; signal transduction; sugars and polysaccharides metabolism
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Lin, S.; Wu, T.; Lin, H.; Zhang, Y.; Xu, S.; Wang, J.; Wu, B.; Chen, Y.; Lin, S.; Lin, D.; Wang, X.; Zhao, X.; Wu, J. De Novo Analysis Reveals Transcriptomic Responses in Eriobotrya japonica Fruits during Postharvest Cold Storage. Genes 2018, 9, 639.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top