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Transcriptome Analysis Reveals Candidate Genes for Cold Tolerance in Drosophila ananassae

Division of Evolutionary Biology, Faculty of Biology, LMU Munich, Grosshaderner Str. 2, 82152 Planegg-Martinsried, Germany
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Genes 2018, 9(12), 624; https://doi.org/10.3390/genes9120624
Received: 27 September 2018 / Revised: 19 November 2018 / Accepted: 3 December 2018 / Published: 12 December 2018
(This article belongs to the Special Issue Evolutionary Genetics of Gene Expression)
Coping with daily and seasonal temperature fluctuations is a key adaptive process for species to colonize temperate regions all over the globe. Over the past 18,000 years, the tropical species Drosophila ananassae expanded its home range from tropical regions in Southeast Asia to more temperate regions. Phenotypic assays of chill coma recovery time (CCRT) together with previously published population genetic data suggest that only a small number of genes underlie improved cold hardiness in the cold-adapted populations. We used high-throughput RNA sequencing to analyze differential gene expression before and after exposure to a cold shock in coldtolerant lines (those with fast chill coma recovery, CCR) and cold-sensitive lines (slow CCR) from a population originating from Bangkok, Thailand (the ancestral species range). We identified two candidate genes with a significant interaction between cold tolerance and cold shock treatment: GF14647 and GF15058. Further, our data suggest that selection for increased cold tolerance did not operate through the increased activity of heat shock proteins, but more likely through the stabilization of the actin cytoskeleton and a delayed onset of apoptosis. View Full-Text
Keywords: chill coma recovery time; cold tolerance; adaptation; Drosophila ananassae; RNA sequencing; heat shock proteins; actin polymerization; apoptosis chill coma recovery time; cold tolerance; adaptation; Drosophila ananassae; RNA sequencing; heat shock proteins; actin polymerization; apoptosis
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Königer, A.; Grath, S. Transcriptome Analysis Reveals Candidate Genes for Cold Tolerance in Drosophila ananassae. Genes 2018, 9, 624.

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