A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women

Rekker, Kadri; Altmäe, Signe; Suhorutshenko, Marina; Peters, Maire; Martinez-Blanch, Juan F.; Codoñer, Francisco M.; Vilella, Felipe; Simón, Carlos; Salumets, Andres; Velthut-Meikas, Agne

doi:10.3390/genes9120574

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A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women

^1,2,*,†,

^2,3,†

^1,2,

^1,2,

Juan F. Martinez-Blanch

⁴,

⁴,

^5,6,

^5,6,7,

^1,2,8,9 and

^2,10

Institute of Clinical Medicine, Department of Obstetrics and Gynecology, University of Tartu, 50406 Tartu, Estonia

Competence Centre on Health Technologies, 50410 Tartu, Estonia

Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of Granada, 18071 Granada, Spain

⁴

Lifesequencing SL, 46980 Valencia, Spain

⁵

Igenomix Foundation/INCLIVA, 46010 Valencia, Spain

⁶

Igenomix SL, 46980 Valencia, Spain

⁷

Department of Obstetrics and Gynecology, Valencia University, 46010 Valencia, Spain

⁸

Institute of Biomedicine and Translational Medicine, Department of Biomedicine, University of Tartu, 50412 Tartu, Estonia

⁹

Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, FI-00014 Helsinki, Finland

¹⁰

Department of Chemistry and Biotechnology, Tallinn University of Technology, 12618 Tallinn, Estonia

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Genes 2018, 9(12), 574; https://doi.org/10.3390/genes9120574

Received: 25 September 2018 / Revised: 7 November 2018 / Accepted: 7 November 2018 / Published: 23 November 2018

(This article belongs to the Special Issue microRNA Regulation in Health and Disease)

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Abstract

The endometrium undergoes extensive changes to prepare for embryo implantation and microRNAs (miRNAs) have been described as playing a significant role in the regulation of endometrial receptivity. However, there is no consensus about the miRNAs involved in mid-secretory endometrial functions. We analysed the complete endometrial miRNome from early secretory (pre-receptive) and mid-secretory (receptive) phases from fertile women and from patients with recurrent implantation failure (RIF) to reveal differentially expressed (DE) miRNAs in the mid-secretory endometrium. Furthermore, we investigated whether the overall changes during early to mid-secretory phase transition and with RIF condition could be reflected in blood miRNA profiles. In total, 116 endometrial and 114 matched blood samples collected from two different population cohorts were subjected to small RNA sequencing. Among fertile women, 91 DE miRNAs were identified in the mid-secretory vs. early secretory endometrium, while no differences were found in the corresponding blood samples. The comparison of mid-secretory phase samples between fertile and infertile women revealed 21 DE miRNAs from the endometrium and one from blood samples. Among discovered novel miRNAs, chr2_4401 was validated and showed up-regulation in the mid-secretory endometrium. Besides novel findings, we confirmed the involvement of miR-30 and miR-200 family members in mid-secretory endometrial functions. View Full-Text

Keywords: endometrial receptivity; infertility; microRNA; recurrent implantation failure; small RNA-seq

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Rekker, K.; Altmäe, S.; Suhorutshenko, M.; Peters, M.; Martinez-Blanch, J.F.; Codoñer, F.M.; Vilella, F.; Simón, C.; Salumets, A.; Velthut-Meikas, A. A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women. Genes 2018, 9, 574. https://doi.org/10.3390/genes9120574

AMA Style

Rekker K, Altmäe S, Suhorutshenko M, Peters M, Martinez-Blanch JF, Codoñer FM, Vilella F, Simón C, Salumets A, Velthut-Meikas A. A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women. Genes. 2018; 9(12):574. https://doi.org/10.3390/genes9120574

Chicago/Turabian Style

Rekker, Kadri, Signe Altmäe, Marina Suhorutshenko, Maire Peters, Juan F. Martinez-Blanch, Francisco M. Codoñer, Felipe Vilella, Carlos Simón, Andres Salumets, and Agne Velthut-Meikas. 2018. "A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women" Genes 9, no. 12: 574. https://doi.org/10.3390/genes9120574

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