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Genes 2018, 9(1), 25;

Methods for Using Small Non-Coding RNAs to Improve Recombinant Protein Expression in Mammalian Cells

Biotechnology Core Laboratory, NIDDK, NIH, Bethesda, MD 20892, USA
Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
Author to whom correspondence should be addressed.
Received: 17 November 2017 / Revised: 19 December 2017 / Accepted: 3 January 2018 / Published: 9 January 2018
(This article belongs to the Special Issue Non-coding RNAs)
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The ability to produce recombinant proteins by utilizing different “cell factories” revolutionized the biotherapeutic and pharmaceutical industry. Chinese hamster ovary (CHO) cells are the dominant industrial producer, especially for antibodies. Human embryonic kidney cells (HEK), while not being as widely used as CHO cells, are used where CHO cells are unable to meet the needs for expression, such as growth factors. Therefore, improving recombinant protein expression from mammalian cells is a priority, and continuing effort is being devoted to this topic. Non-coding RNAs are RNA segments that are not translated into a protein and often have a regulatory role. Since their discovery, major progress has been made towards understanding their functions. Non-coding RNA has been investigated extensively in relation to disease, especially cancer, and recently they have also been used as a method for engineering cells to improve their protein expression capability. In this review, we provide information about methods used to identify non-coding RNAs with the potential of improving recombinant protein expression in mammalian cell lines. View Full-Text
Keywords: microRNA; screening; microarray; microRNA library; next generation sequencing microRNA; screening; microarray; microRNA library; next generation sequencing

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Inwood, S.; Betenbaugh, M.J.; Shiloach, J. Methods for Using Small Non-Coding RNAs to Improve Recombinant Protein Expression in Mammalian Cells. Genes 2018, 9, 25.

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