Next Article in Journal
Advances in Genomic Profiling and Analysis of 3D Chromatin Structure and Interaction
Next Article in Special Issue
Emerging Estrogenic Pollutants in the Aquatic Environment and Breast Cancer
Previous Article in Journal
High-Throughput Study of the Effects of Celastrol on Activated Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis
Previous Article in Special Issue
Zebrafish Xenograft: An Evolutionary Experiment in Tumour Biology
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Genes 2017, 8(9), 222;

RECQ1 Helicase Silencing Decreases the Tumour Growth Rate of U87 Glioblastoma Cell Xenografts in Zebrafish Embryos

Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Večna pot 111, 1000 Ljubljana, Slovenia
Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000 Ljubljana, Slovenia
International Postgraduate School Jozef Stefan, Jamova 39, 1000 Ljubljana, Slovenia
Structural Biology Laboratory, Elettra-Sincrotrone Trieste, Strada Statale 14‑km 163, 5, Basovizza, 34149 Trieste, Italy
Department of Biochemistry, Faculty of Chemistry and Chemical Engineering, University of Ljubljana, Večna pot 113, 1000 Ljubljana, Slovenia
Author to whom correspondence should be addressed.
Academic Editor: Laura Sánchez
Received: 14 July 2017 / Revised: 18 August 2017 / Accepted: 5 September 2017 / Published: 6 September 2017
(This article belongs to the Special Issue Zebrafish: The Key for Cancer Treatment)
Full-Text   |   PDF [2738 KB, uploaded 6 September 2017]   |  


RECQ1 helicase has multiple roles in DNA replication, including restoration of the replication fork and DNA repair, and plays an important role in tumour progression. Its expression is highly elevated in glioblastoma as compared to healthy brain tissue. We studied the effects of small hairpin RNA (shRNA)-induced silencing of RECQ1 helicase on the increase in cell number and the invasion of U87 glioblastoma cells. RECQ1 silencing reduced the rate of increase in the number of U87 cells by 30%. This corresponded with a 40% reduction of the percentage of cells in the G2 phase of the cell cycle, and an accumulation of cells in the G1 phase. These effects were confirmed in vivo, in the brain of zebrafish (Danio rerio) embryos, by implanting DsRed-labelled RECQ1 helicase-silenced and control U87 cells. The growth of resulting tumours was quantified by monitoring the increase in xenograft fluorescence intensity during a three-day period with fluorescence microscopy. The reduced rate of tumour growth, by approximately 30% in RECQ1 helicase-silenced cells, was in line with in vitro measurements of the increase in cell number upon RECQ1 helicase silencing. However, RECQ1 helicase silencing did not affect invasive behaviour of U87 cells in the zebrafish brain. This is the first in vivo confirmation that RECQ1 helicase is a promising molecular target in the treatment of glioblastoma. View Full-Text
Keywords: cancer; cell cycle; DNA damage; intravital imaging; RNA interference; theranostics cancer; cell cycle; DNA damage; intravital imaging; RNA interference; theranostics

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Vittori, M.; Breznik, B.; Hrovat, K.; Kenig, S.; Lah, T.T. RECQ1 Helicase Silencing Decreases the Tumour Growth Rate of U87 Glioblastoma Cell Xenografts in Zebrafish Embryos. Genes 2017, 8, 222.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top