Mapping of QTLs for Seed Phorbol Esters, a Toxic Chemical in Jatropha curcas (L.)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and DNA Extraction
2.2. Quantification of Phorbol Esters Content in Seeds
2.3. SSR Marker Analysis
2.4. Linkage Map Construction and QTL Analysis
2.5. Identification of Physical Locations of Markers Flanking QTLs and Genes Associated with QTL
3. Results
3.1. Variation of Seed PE in Parents and F2 Population
3.2. SSR Polymorphism and Linkage Map
3.3. QTL Controlling Seed PE in Jatropha
3.4. Physical Location of the PE QTLs and Annotated Genes in the QTL Regions
4. Discussion
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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LG a | Marker Name | R2 (%) b | Position | LOD Score |
---|---|---|---|---|
3 | CN_SSR199 | 11.74 | 145.32 | 2.58 |
3 | CN_SSR327 | 12.25 | 150.18 | 2.70 |
3 | CN_SSR326 | 15.88 | 152.84 | 3.57 |
3 | CN_SSR330 | 11.67 | 165.73 | 2.56 |
3 | CN_SSR336 | 12.88 | 198.11 | 2.84 |
8 | NG291 | 14.20 | 47.11 | 3.16 |
8 | NG288C | 13.06 | 48.22 | 2.89 |
8 | NG286A | 16.99 | 50.41 | 3.84 |
8 | MPN280 | 16.40 | 51.48 | 3.70 |
8 | G262 | 13.55 | 54.15 | 3.00 |
8 | G270B | 15.69 | 55.21 | 3.52 |
8 | G270D | 15.64 | 57.34 | 3.51 |
8 | G282B | 13.94 | 59.47 | 3.10 |
8 | G273A | 15.57 | 62.14 | 3.49 |
QTL Name | LG a | Marker Interval | Position (cM) b | LOD Score | Confidence Interval (cM) c | PVE (%) d | Additive Effect e | Dominant Effect | [d/a] f |
---|---|---|---|---|---|---|---|---|---|
qPE3.1 | 3 | CN_SSR326–CN_SSR325 | 153 | 3.82 | 152.5–156.5 | 14.10 | −0.09 | −0.41 | 4.56 |
qPE8.1 | 8 | NG288C–NG286A | 50 | 4.06 | 48.5–51.5 | 15.49 | 0.31 | 0.02 | 0.06 |
qPE3.1 | ||||
Name | Position | Annotation | GO Term | KEGG |
LOC105634082 | NW_012124225.1:1070722..1072343 | Uncharacterized protein At4g22758 | - | - |
qPE8.1 | ||||
Name | Position | Annotation | GO Term | KEGG |
LOC105650633 | NW_012130064.1:251091..252362 | Peptidyl-prolylcis-trans isomerase CYP26-2, chloroplastic | GO:0003755 | K03768 |
LOC105650634 | NW_012130064.1:244937..251195 | Uncharacterized LOC105650634 | GO:0008270 | K03768 |
LOC105650635 | NW_012130064.1:253456..257226 | Single-stranded DNA-binding protein, mitochondrial | GO:0003697 | K03111 |
LOC105650637 | NW_012130064.1:259463..262260 | Uncharacterized LOC105650637 | - | - |
LOC105650638 | NW_012130064.1:262323..266102 | Uncharacterized LOC105650638 | - | - |
LOC105650639 | NW_012130064.1:267598..271421 | Zinc finger BED domain-containing protein DAYSLEEPER | GO:0003677, GO:0046983, GO:0003676 | K03680 |
LOC105650640 | NW_012130064.1:273262..278864 | Translation initiation factor eIF-2B subunit delta | GO:0044237 | K03680 |
LOC105650641 | NW_012130064.1:288075..288870 | Oleosin 1-like | GO:0016021, GO:0012511 | - |
LOC105650642 | NW_012130064.1:291937..292732 | Uncharacterized LOC105650642 | - | K13111 |
LOC105650712 | NW_012130064.1:279771..287264 | Pseudogene | - | - |
LOC105650643 | NW_012130064.1:297597..314028 | Suppressor of mec-8 and unc-52 protein homolog 1 | GO:0005515 | K13111 |
LOC105650713 | NW_012130064.1:317012..317392 | Inactive protein FON2 SPARE1-like | - | - |
LOC105650644 | NW_012130064.1:324023..347807 | Transcription initiation factor TFIID subunit 2 | GO:0008237, GO:0008270, GO:0005488 | K03128 |
LOC105650714 | NW_012130064.1:367045..368556 | Transcription factor CYCLOIDEA | - | K08735 |
LOC105650645 | NW_012130064.1:376579..386656 | DNA mismatch repair protein MSH2 | GO:0005524, GO:0030983, GO:0006298 | K08735 |
LOC105650646 | NW_012130064.1:392247..402394 | Uncharacterized LOC105650646 | GO:0005515, GO:0008270 | - |
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Amkul, K.; Laosatit, K.; Somta, P.; Shim, S.; Lee, S.-H.; Tanya, P.; Srinives, P. Mapping of QTLs for Seed Phorbol Esters, a Toxic Chemical in Jatropha curcas (L.). Genes 2017, 8, 205. https://doi.org/10.3390/genes8080205
Amkul K, Laosatit K, Somta P, Shim S, Lee S-H, Tanya P, Srinives P. Mapping of QTLs for Seed Phorbol Esters, a Toxic Chemical in Jatropha curcas (L.). Genes. 2017; 8(8):205. https://doi.org/10.3390/genes8080205
Chicago/Turabian StyleAmkul, Kitiya, Kularb Laosatit, Prakit Somta, Sangrea Shim, Suk-Ha Lee, Patcharin Tanya, and Peerasak Srinives. 2017. "Mapping of QTLs for Seed Phorbol Esters, a Toxic Chemical in Jatropha curcas (L.)" Genes 8, no. 8: 205. https://doi.org/10.3390/genes8080205