Variation in the Ovine KAP6-3 Gene (KRTAP6-3) Is Associated with Variation in Mean Fibre Diameter-Associated Wool Traits
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sheep Blood and Wool Samples
2.2. PCR Primers and Amplification
2.3. Screening for Variation in KRTAP6-3
2.4. Sequencing of Allelic Variants and Sequence Analysis
2.5. Statistical Analyses
3. Results
3.1. Variation in Ovine KRTAP6-3
3.2. Associations between Variation in KRTAP6-3 and Wool Traits
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Trait 1 | Mean ± SE 2 | p3 | ||
---|---|---|---|---|
AA (n = 238) | AB (n = 17) | AG (n = 105) | ||
GFW (kg) | 2.14 ± 0.11 | 2.23 ± 0.15 | 2.11 ± 0.12 | 0.631 |
CFW (kg) | 1.62 ± 0.09 | 1.66 ± 0.12 | 1.57 ± 0.10 | 0.454 |
Yield (%) | 75.4 ± 1.63 | 74.3 ± 2.18 | 74.0 ± 1.81 | 0.220 |
MSL (mm) | 85.0 ± 3.23 | 83.9 ± 4.32 | 86.9 ± 3.60 | 0.483 |
MSS (N/ktex) | 21.5 ± 2.13 | 22.7 ± 2.84 | 21.0 ± 2.36 | 0.732 |
MFD (µm) | 19.0 ± 0.46 b | 20.8 ± 0.61 a | 18.1 ± 0.51 c | <0.001 |
FDSD (µm) | 4.04 ± 0.16 b | 4.41 ± 0.21 a | 3.79 ± 0.18 c | <0.001 |
CVFD (%) | 21.2 ± 0.59 | 21.1 ± 0.79 | 20.8 ± 0.66 | 0.552 |
MFC (°/mm) | 89.1 ± 4.20 | 91.4 ± 5.61 | 85.3 ± 4.66 | 0.197 |
PF (%) | 1.89 ± 0.79 b | 4.88 ± 1.06 a | 0.76 ± 0.88 c | <0.001 |
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Li, S.; Zhou, H.; Gong, H.; Zhao, F.; Wang, J.; Luo, Y.; Hickford, J.G.H. Variation in the Ovine KAP6-3 Gene (KRTAP6-3) Is Associated with Variation in Mean Fibre Diameter-Associated Wool Traits. Genes 2017, 8, 204. https://doi.org/10.3390/genes8080204
Li S, Zhou H, Gong H, Zhao F, Wang J, Luo Y, Hickford JGH. Variation in the Ovine KAP6-3 Gene (KRTAP6-3) Is Associated with Variation in Mean Fibre Diameter-Associated Wool Traits. Genes. 2017; 8(8):204. https://doi.org/10.3390/genes8080204
Chicago/Turabian StyleLi, Shaobin, Huitong Zhou, Hua Gong, Fangfang Zhao, Jiqing Wang, Yuzhu Luo, and Jon G. H. Hickford. 2017. "Variation in the Ovine KAP6-3 Gene (KRTAP6-3) Is Associated with Variation in Mean Fibre Diameter-Associated Wool Traits" Genes 8, no. 8: 204. https://doi.org/10.3390/genes8080204