Next Article in Journal
Evolutionary Origins of Cancer Driver Genes and Implications for Cancer Prognosis
Next Article in Special Issue
Time to Spread Your Wings: A Review of the Avian Ancient DNA Field
Previous Article in Journal
Special Issue Introduction: Role of Epigenetic Gene Regulation in Brain Function
Previous Article in Special Issue
Identifying Bird Remains Using Ancient DNA Barcoding
Open AccessReview

Novel Substrates as Sources of Ancient DNA: Prospects and Hurdles

BioArCh, Department of Archaeology, University of York, Wentworth Way, York YO10 5DD, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Michael Hofreiter
Genes 2017, 8(7), 180; https://doi.org/10.3390/genes8070180
Received: 30 May 2017 / Revised: 22 June 2017 / Accepted: 10 July 2017 / Published: 13 July 2017
(This article belongs to the Special Issue Novel and Neglected Areas of Ancient DNA Research)
Following the discovery in the late 1980s that hard tissues such as bones and teeth preserve genetic information, the field of ancient DNA analysis has typically concentrated upon these substrates. The onset of high-throughput sequencing, combined with optimized DNA recovery methods, has enabled the analysis of a myriad of ancient species and specimens worldwide, dating back to the Middle Pleistocene. Despite the growing sophistication of analytical techniques, the genetic analysis of substrates other than bone and dentine remain comparatively “novel”. Here, we review analyses of other biological substrates which offer great potential for elucidating phylogenetic relationships, paleoenvironments, and microbial ecosystems including (1) archaeological artifacts and ecofacts; (2) calcified and/or mineralized biological deposits; and (3) biological and cultural archives. We conclude that there is a pressing need for more refined models of DNA preservation and bespoke tools for DNA extraction and analysis to authenticate and maximize the utility of the data obtained. With such tools in place the potential for neglected or underexploited substrates to provide a unique insight into phylogenetics, microbial evolution and evolutionary processes will be realized. View Full-Text
Keywords: ancient DNA; methodological advances; PCR; NGS ancient DNA; methodological advances; PCR; NGS
Show Figures

Figure 1

MDPI and ACS Style

Green, E.J.; Speller, C.F. Novel Substrates as Sources of Ancient DNA: Prospects and Hurdles. Genes 2017, 8, 180.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop