Elaborated Action of the Human Primosome
AbstractThe human primosome is a 340-kilodalton complex of primase (DNA-dependent RNA polymerase) and DNA polymerase α, which initiates genome replication by synthesizing chimeric RNA-DNA primers for DNA polymerases δ and ϵ. Accumulated biochemical and structural data reveal the complex mechanism of concerted primer synthesis by two catalytic centers. First, primase generates an RNA primer through three steps: initiation, consisting of dinucleotide synthesis from two nucleotide triphosphates; elongation, resulting in dinucleotide extension; and termination, owing to primase inhibition by a mature 9-mer primer. Then Polα, which works equally well on DNA:RNA and DNA:DNA double helices, intramolecularly catches the template primed by a 9mer RNA and extends the primer with dNTPs. All primosome transactions are highly coordinated by autoregulation through the alternating activation/inhibition of the catalytic centers. This coordination is mediated by the small C-terminal domain of the primase accessory subunit, which forms a tight complex with the template:primer, shuttles between the primase and DNA polymerase active sites, and determines their access to the substrate. View Full-Text
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Baranovskiy, A.G.; Tahirov, T.H. Elaborated Action of the Human Primosome. Genes 2017, 8, 62.
Baranovskiy AG, Tahirov TH. Elaborated Action of the Human Primosome. Genes. 2017; 8(2):62.Chicago/Turabian Style
Baranovskiy, Andrey G.; Tahirov, Tahir H. 2017. "Elaborated Action of the Human Primosome." Genes 8, no. 2: 62.
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