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10.3390/genes8120391
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Article

Isolation and Characterization of Ftsz Genes in Cassava

by
Meng-Ting Geng
1,2,†,
Yi Min
2,†,
Yuan Yao
1,†,
Xia Chen
2,
Jie Fan
2,
Shuai Yuan
2,
Lei Wang
2,
Chong Sun
3,
Fan Zhang
2,
Lu Shang
2,
Yun-Lin Wang
2,
Rui-Mei Li
1,
Shao-Ping Fu
1,
Rui-Jun Duan
1,
Jiao Liu
1,
Xin-Wen Hu
2,* and
Jian-Chun Guo
1,*
1
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
2
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
3
College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Genes 2017, 8(12), 391; https://doi.org/10.3390/genes8120391
Submission received: 18 October 2017 / Revised: 1 December 2017 / Accepted: 12 December 2017 / Published: 15 December 2017
(This article belongs to the Section Plant Genetics and Genomics)
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Abstract

The filamenting temperature-sensitive Z proteins (FtsZs) play an important role in plastid division. In this study, three FtsZ genes were isolated from the cassava genome, and named MeFtsZ1, MeFtsZ2-1, and MeFtsZ2-2, respectively. Based on phylogeny, the MeFtsZs were classified into two groups (FtsZ1 and FtsZ2). MeFtsZ1 with a putative signal peptide at N-terminal, has six exons, and is classed to FtsZ1 clade. MeFtsZ2-1 and MeFtsZ2-2 without a putative signal peptide, have seven exons, and are classed to FtsZ2 clade. Subcellular localization found that all the three MeFtsZs could locate in chloroplasts and form a ring in chloroplastids. Structure analysis found that all MeFtsZ proteins contain a conserved guanosine triphosphatase (GTPase) domain in favor of generate contractile force for cassava plastid division. The expression profiles of MeFtsZ genes by quantitative reverse transcription-PCR (qRT-PCR) analysis in photosynthetic and non-photosynthetic tissues found that all of the MeFtsZ genes had higher expression levels in photosynthetic tissues, especially in younger leaves, and lower expression levels in the non-photosynthetic tissues. During cassava storage root development, the expressions of MeFtsZ2-1 and MeFtsZ2-2 were comparatively higher than MeFtsZ1. The transformed Arabidopsis of MeFtsZ2-1 and MeFtsZ2-2 contained abnormally shape, fewer number, and larger volume chloroplasts. Phytohormones were involved in regulating the expressions of MeFtsZ genes. Therefore, we deduced that all of the MeFtsZs play an important role in chloroplast division, and that MeFtsZ2 (2-1, 2-2) might be involved in amyloplast division and regulated by phytohormones during cassava storage root development.
Keywords: isolation; FtsZ; cassava; subcellular localization; gene expression; phytohormone response; plastid division; transgenic Arabidopsis isolation; FtsZ; cassava; subcellular localization; gene expression; phytohormone response; plastid division; transgenic Arabidopsis

Share and Cite

MDPI and ACS Style

Geng, M.-T.; Min, Y.; Yao, Y.; Chen, X.; Fan, J.; Yuan, S.; Wang, L.; Sun, C.; Zhang, F.; Shang, L.; et al. Isolation and Characterization of Ftsz Genes in Cassava. Genes 2017, 8, 391. https://doi.org/10.3390/genes8120391

AMA Style

Geng M-T, Min Y, Yao Y, Chen X, Fan J, Yuan S, Wang L, Sun C, Zhang F, Shang L, et al. Isolation and Characterization of Ftsz Genes in Cassava. Genes. 2017; 8(12):391. https://doi.org/10.3390/genes8120391

Chicago/Turabian Style

Geng, Meng-Ting, Yi Min, Yuan Yao, Xia Chen, Jie Fan, Shuai Yuan, Lei Wang, Chong Sun, Fan Zhang, Lu Shang, and et al. 2017. "Isolation and Characterization of Ftsz Genes in Cassava" Genes 8, no. 12: 391. https://doi.org/10.3390/genes8120391

APA Style

Geng, M.-T., Min, Y., Yao, Y., Chen, X., Fan, J., Yuan, S., Wang, L., Sun, C., Zhang, F., Shang, L., Wang, Y.-L., Li, R.-M., Fu, S.-P., Duan, R.-J., Liu, J., Hu, X.-W., & Guo, J.-C. (2017). Isolation and Characterization of Ftsz Genes in Cassava. Genes, 8(12), 391. https://doi.org/10.3390/genes8120391

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