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Genes 2017, 8(11), 338;

Connections between Transcription Downstream of Genes and cis-SAGe Chimeric RNA

Department of Pathology, School of Medicine, University of Virginia, Charlottesville, VA 22908, USA
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia, Charlottesville, VA 22908, USA
Author to whom correspondence should be addressed.
Received: 16 October 2017 / Revised: 10 November 2017 / Accepted: 16 November 2017 / Published: 22 November 2017
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cis-Splicing between adjacent genes (cis-SAGe) is being recognized as one way to produce chimeric fusion RNAs. However, its detail mechanism is not clear. Recent study revealed induction of transcriptions downstream of genes (DoGs) under osmotic stress. Here, we investigated the influence of osmotic stress on cis-SAGe chimeric RNAs and their connection to DoGs. We found, the absence of induction of at least some cis-SAGe fusions and/or their corresponding DoGs at early time point(s). In fact, these DoGs and their cis-SAGe fusions are inversely correlated. This negative correlation was changed to positive at a later time point. These results suggest a direct competition between the two categories of transcripts when total pool of readthrough transcripts is limited at an early time point. At a later time point, DoGs and corresponding cis-SAGe fusions are both induced, indicating that total readthrough transcripts become more abundant. Finally, we observed overall enhancement of cis-SAGe chimeric RNAs in KCl-treated samples by RNA-Seq analysis. View Full-Text
Keywords: DoG; Cis-SAGe; chimeric RNA; osmotic stress DoG; Cis-SAGe; chimeric RNA; osmotic stress

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Chwalenia, K.; Qin, F.; Singh, S.; Tangtrongstittikul, P.; Li, H. Connections between Transcription Downstream of Genes and cis-SAGe Chimeric RNA. Genes 2017, 8, 338.

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