Correction: Garbuz et al. Design, Optimization and Validation of the ARMS PCR Protocol for the Rapid Diagnosis of Wilson’s Disease Using a Panel of 14 Common Mutations for the European Population. Genes 2022, 13, 1940

Mikhail Maksimovich Garbuz; Anna Alexandrovna Ovchinnikova; Vadim Vladimirovich Kumeiko

doi:10.3390/genes15081070

,

and

¹

Institute of Life Sciences and Biomedicine, School of Natural Sciences, Far Eastern Federal University, Vladivostok 690922, Russia

²

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch of Russian Academy of Sciences, Federal University, Vladivostok 690041, Russia

^*

Author to whom correspondence should be addressed.

Genes2024, 15(8), 1070;https://doi.org/10.3390/genes15081070

This article belongs to the Section Genetic Diagnosis

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In the original publication [1], the affiliation 1 was updated. There was also a mistake in Tables 1 and 2 as published. The errors were technical and occurred while transferring data into a table using a template. To avoid problems with reproducibility, and the possibility of our method being used by interested people, we request that these changes to be made to Tables 1 and 2. The corrected Table 1 and Table 2 appear below. The authors confirm that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.

Table 1. Primers for exon amplification of the ATP7B gene.

Table 2. Primers for allele-specific PCR of the ATP7B mutation sites.

Reference

Garbuz, M.M.; Ovchinnikova, A.A.; Kumeiko, V.V. Design, Optimization and Validation of the ARMS PCR Protocol for the Rapid Diagnosis of Wilson’s Disease Using a Panel of 14 Common Mutations for the European Population. Genes 2022, 13, 1940. [Google Scholar] [CrossRef] [PubMed]

Table 1. Primers for exon amplification of the ATP7B gene.

Exon	Forward Primer	Revers Primer
2A	GGCATTGTTTTCCATTTTCTCAGTG	CATGTCCCCAATTTGATGGCAAAC
2B	GAAGGTTTCCCTGGAACAAGGC	GCAGAAGATAAAGGTCTCTTTGGG
2C	TGGGACCAATTGATATTGAGCGG	GGAAGACCTGTGATCTGTCCC
2D	TATCGAGGCACTTCCACCTGG	CTCACCTATACCACCATCCAGG
3	GGTGGGAGCCGGGACAATGAACC	CAGCATTCCTAAGTTCAACATGGG
4	TATTGACTGTGTCAACCTAGAGGC	AAACTGTCAGAAGCCTGTAACCC
5	CTGCCTGTTACCTAGACTCCC	TTACCCATTCACTGATATCCTCCC
6	AAAACCCACAAAGTCTACTGAGGC	CAGCTAATCCAGGAGGAAGGC
7	TCTTAAACTGTGTCCTCAGAAGGG	ACTATGTTTGCGCTTAGCGGGC
8	GACTGTGCACAAAGCTAGAGGC	GAGATTTGTTTACTGAAGGAGCAGC
9	GTGTGGTGGATAGCAAGTAACGC	CTTTCGTAGCTGGATTGAGAGTGG
10 + 11	AGCTGGCCTAGAACCTGACCC	GAACTCTTCACATAATTTCTAAAACGAGA
12	CCCAATCTTTATCCATGCTTGTGG	AGTGACTGTTTATCCTACTCTGGC
13	CCTTATTGAACTCTCAACCTGCC	CTCTGTTGCTACTGTTGTTATTCCC
14	CCCTGAGATTGAACGACAGAGG	GGTGAGGAATAAAAGAGCATTGGC
15	CTTTCCGCTGCTCTCTTGCC	CAGAGGCAATCACTGCTGGG
16	TGTCACAAGAGGTGCTTACAAGG	GCCTGAAATTAAGAGAGGAAGGC
17	CTTCCAGACTTTTGTGTACATCCG	GAGTACAGCTCAGTGCTGGG
18 + 19	TTTTGCCAACACTAGGCATTGCC	GGAGACAGAAGCCTTTCTGGG
20	GAACATCAGGGCGAGTGGAAG	GTGCTAAGCATGCAGAATGACAAG

Table 2. Primers for allele-specific PCR of the ATP7B mutation sites.

Exon	Mutation	Mutant forward Primer	Normal forward Primer	Revers Primer
5	Arg616Gln	TTTGACCCGGAAATTATCGGTCCATA	GACCCGGAAATTATCGGTCCATG	CAAGCTCTCCACAACAAGAGTGG
8	Met769HisfsTer26	CATTCTTCGACACGCCCCCTC	ACATTCTTCGACACGCCCCCTA	TTTGGAGATTAGTGACTAGAGCACCT
	Gly710Ser	CTGTCCTTGTCTTTCAGCTCCTTA	CTGTCCTTGTCTTTCAGCTCCTTG
	Ser744Pro	GGCCACAAGCATTGCTTATGTTTTTC	TGGCCACAAGCATTGCTTATGTTTTTT
	Arg778Leu	CTTTGTGTTCATTGCCCTGGGTCT	TTTGTGTTCATTGCCCTGGGTCG	GACCAACTACATATTCAGTTTTGCACC
	Arg778Gly	CTTTGTGTTCATTGCCCTGGGTG	CTTTGTGTTCATTGCCCTGGGTC
	Trp779*	TCATTGCCCTGGGCCGGTCA	TTCATTGCCCTGGGCCGGTTG
10	Val834Asp	GGGGCGATATCGTCAAGGTGTA	GGGGCGATATCGTCAAGGTGTT	GTCTGATTTCCCAGAACTCTTCACATA
12	Gly943Ser	TTGACGTTGGTGGTATGGATTGTAATTA	GACGTTGGTGGTATGGATTGTAATTG	CAACTGAGCACCAATTGGTGTCTG
14	His1069Gln	TGCGGAGGCCAGCAGTGAATAA	GCGGAGGCCAGCAGTGAATAC	AGACTGCCCGTACTCCCCAAG
	Glu1064Lys	GGCTGTGGTGGGGACTGCTA	GCTGTGGTGGGGACTGCTG
	Arg1041Trp	GGCGTCCCCAGGGTCACGT	GCGTCCCCAGGGTCACGC
	3222_3243+21del43	GGCGTGGCGTACGTGGACTT	GTGGCAGTCACCAAATACTGTAAAG
15	Ala1135GlnfsTer13	TGAGGCTGGCAGCCTTCCCTA	AGGCTGGCAGCCTTCCCTC	ACATTTCCCTCTGCTTTCCCTGAC

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Correction: Garbuz et al. Design, Optimization and Validation of the ARMS PCR Protocol for the Rapid Diagnosis of Wilson’s Disease Using a Panel of 14 Common Mutations for the European Population. Genes 2022, 13, 1940

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